Generation of the Rat Monoclonal Antibody Against the Extracellular Domain of Human CD63 by DNA Immunization
Generation of the Rat Monoclonal Antibody Against the Extracellular Domain of Human CD63 by DNA Immunization
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DNA免疫产生抗人CD63胞外结构域的大鼠单克隆抗体
DOI：
10.1089/mab.2020.0007
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发表时间：
2020
影响因子：
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通讯作者：
Tachibana Taro
中科院分区：
文献类型：
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作者：
Xu Liu;Ihara Kan-ichiro;Yoshimura Saori;Konno Daijiro;Tachibana Akira;Nakanishi Takeshi;Tachibana Taro
人分化簇63（Human cluster of differentiation 63，hCD 63）是一种广泛存在于外泌体表面的四跨膜蛋白受体。外泌体参与细胞与细胞的通讯，包括从癌细胞到正常细胞。检测外泌体作为诊断各种疾病的标志物是非常重要的。在这项研究中，我们报告的单克隆抗体（mAb）的产生和表征的细胞外结构域的hCD 63使用DNA免疫。该mAb（克隆1C 8 - 2B 11）在免疫荧光和流式细胞术中表现出高性能，其亲和力是市售对照抗体的10倍。mAb 1C 8 - 2B 11具有很大的潜力，可作为研究和临床诊断的工具。
Human cluster of differentiation 63 (hCD63) is one of the tetraspanin receptors that is abundant on the surface of exosomes. Exosomes are involved in cell-to-cell communication, including from cancer cells to normal cells. It is very important to detect exosomes as a marker for the diagnosis of various diseases. In this study, we report the generation and characterization of a monoclonal antibody (mAb) against the extracellular domain of hCD63 using DNA immunization. This mAb, clone 1C8-2B11, exhibits high performance for use in immunofluorescence and flow cytometry, and it has 10-fold higher affinity than the control antibody that is commercially available. mAb 1C8-2B11 has great potential to be a tool for research and clinical diagnosis.