Structure and function of a small RNA that selectively inhibits internal ribosome entry site-mediated translation.
Structure and function of a small RNA that selectively inhibits internal ribosome entry site-mediated translation.
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选择性抑制内部核糖体进入位点介导翻译的小 RNA 的结构和功能。
DOI：
10.1093/nar/27.2.562
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发表时间：
1999
影响因子：
14.9
通讯作者：
Dasgupta,A
中科院分区：
文献类型：
--
作者：
Venkatesan,A;Das,S;Dasgupta,A
60 nt long RNA termed IRNA, isolated from the yeastSaccharomyces cerevesiae, was previously shown to selectively block internal ribosome entry site (IRES)-mediated translation without interfering with cap-dependent translation of cellular mRNAs bothin vivoandin vitro. IRNA specifically bound cellular proteins believed to be important for IRES-mediated translation. We demonstrate here that a complementary copy of IRNA (cIRNA) is also active in blocking IRES-mediated translation and that it binds many of the same cellular proteins that IRNA does. We have probed the secondary structure of both IRNA and cIRNA using single-strand- and double-strand-specific nucleases as well as using oligonucleotide hybridization followed by RNase H digestion. Both IRNA and cIRNA share secondary structural homology, although distinct differences do exist between the two structures. Mutational analysis of IRNA shows that sequences that form both the main stem and one loop are critical for its translation inhibitory activity. Maintenance of the established secondary structure appears to be required for both IRNA's ability to bind cellular trans-acting proteins believed to be required for IRES-mediated translation and its ability to block IRES-mediated translation.
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使用通过引物延伸监测的化学和酶探测对 RNA 进行结构分析。
影响因子：
--
作者：
S. Stern;D. Moazed;H. Noller
通讯作者：
H. Noller
脊髓灰质炎病毒RNA 5非编码区的体内遗传变异和拟议的功能域
影响因子：
5.4
作者：
T. Pöyry;L. Kinnunen;T. Hovi
通讯作者：
T. Hovi
DOI：
10.1073/pnas.90.16.7642
发表时间：
1993-08-15
影响因子：
11.1
作者：
HELLEN, CUT;WITHERELL, GW;WIMMER, E
通讯作者：
WIMMER, E
影响因子：
5.6
作者：
MOAZED, D;STERN, S;NOLLER, HF
通讯作者：
NOLLER, HF
影响因子：
11.4
作者：
GUO, HCT;COLLINS, RA
通讯作者：
COLLINS, RA