Short Report: Giardia intestinalis Assemblages A and B Infections in Nepal
Short Report: Giardia intestinalis Assemblages A and B Infections in Nepal
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DOI：
10.4269/ajtmh.2009.81.538
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发表时间：
2009-09-01
影响因子：
3.3
通讯作者：
Houpt, Eric R.
中科院分区：
文献类型：
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作者：
Singh, Anjana;Janaki, Lalitha;Houpt, Eric R.
贾第鞭毛虫由两种主要的基因型A和B组成，这两种基因型在致病倾向上可能不同。我们检测了尼泊尔胃肠道症状患者粪便样本中这两种基因型的存在。共对1,096份临床标本进行了镜检，其中45份标本为G.确定了可疑人员。贾第鞭毛虫感染证实了35的45个样本的贾第鞭毛虫特异性实时聚合酶链反应（PCR）检测。应用限制性片段长度多态性对贾第虫PCR产物进行基因分型，结果显示74%（26/35）为集合B，20%（7/35）为集合A，6%（2/35）为混合集合。
Giardia intestinalis is comprised of two major genotypes, A and B, which may vary in their propensity to cause disease. We tested for the presence of these two genotypes in stool samples from patients with gastrointestinal symptoms in Nepal. A total of 1,096 clinical specimens were screened by microscopy, and 45 samples with G. intestinalis were identified. Giardia infection was confirmed in 35 of 45 samples by a Giardia specific real-time polymerase chain reaction (PCR) assay. Genotyping of the Giardia PCR product by restriction fragment length polymorphism indicated that 74% (26 of 35) were assemblage B, 20% (7 of 35) were assemblage A, and 6% (2 of 35) were mixed assemblages.