Age-dependent changes in spontaneous frequency of micronucleated erythrocytes in bone marrow and DNA damage in peripheral blood of Swiss mice
Age-dependent changes in spontaneous frequency of micronucleated erythrocytes in bone marrow and DNA damage in peripheral blood of Swiss mice
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DOI：
10.1016/j.mrgentox.2014.04.026
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发表时间：
2014-08-01
影响因子：
1.9
通讯作者：
Chaubey, R. C.
中科院分区：
文献类型：
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作者：
Bhilwade, Hari N.;Jayakumar, S.;Chaubey, R. C.
对雄性和雌性瑞士小鼠的骨髓(一种自我增殖的组织)中自发出现的微核形式的染色体损伤和外周血中DNA损伤的年龄相关性变化进行了研究。在小鼠骨髓的红细胞群中，多染(未成熟)红细胞的微核率随年龄(1-20个月)的增加而显著增加。正常(成熟)红细胞微核率增加较少。在所检测的所有年龄组中，雌性小鼠的微核率高于雄性小鼠。但总红细胞和多染红细胞微核率的雌雄比随年龄增长而降低。不同年龄组(1、6、12、18月龄)小鼠外周血DNA损伤以单细胞凝胶电泳法测定的尾矩为指标，随增龄逐渐增加。雌性小鼠表现出比1月龄和18月龄雄性小鼠更大的DNA损伤。综上所述，这些结果表明，在衰老过程中，小鼠骨髓中的遗传损伤和外周血中的DNA损伤都在积累，并且雌性比雄性表现出更多的变化。(C)2014爱思唯尔B.V.保留所有权利。
Age-dependent changes in chromosomal damage in bone marrow - a self-proliferating tissue - in the form of spontaneously occurring micronucleated erythrocytes, and DNA damage in peripheral blood were examined in male and female Swiss mice. In the erythrocyte population in the bone marrow, polychromatic (immature) erythrocytes showed a significant increase in the frequency of micronuclei as a function of age of the mice (1-20 months). The increase in micronucleus frequency was less in normochromatic (mature) erythrocytes. The female mice showed a higher frequency of micronuclei than the male mice in all the age groups examined. However, the female to male ratio of micronucleus frequencies in total erythrocytes as well as in polychromatic erythrocytes decreased with age. DNA damage, measured as tail moment in the single-cell gel electrophoresis in peripheral blood of different age groups of mice (1, 6, 12 and 18 months) showed a gradual increase with age. Female mice showed more DNA damage than 1-month and 18-month-old male mice. In conclusion, these results show that there is an accumulation of genetic damage in bone marrow and DNA damage in peripheral blood of mice during ageing, and that females show more alterations than males. (C) 2014 Elsevier B.V. All rights reserved.