Label Free Particle-by-Particle Quantification of DNA Loading on Sorted Gold Nanostars
Label Free Particle-by-Particle Quantification of DNA Loading on Sorted Gold Nanostars
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DOI：
10.1021/acs.analchem.8b03715
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发表时间：
2019-05-07
影响因子：
7.4
通讯作者：
Schweikert, Emile A.
中科院分区：
文献类型：
--
作者：
Eller, Michael J.;Chandra, Kavita;Schweikert, Emile A.
本文介绍了一种无标记技术，用于确定金属纳米粒子上的配体负载使用的二次离子质谱的变体。Au-400（4+）簇轰击具有相似表面积的DNA官能化的各向异性金纳米星和各向同性纳米球以确定配体密度。对于每个射弹撞击，检查每个颗粒在单次撞击（直径10-15 nm）的发射区域内的共定位分子。单个纳米颗粒分析允许确定颗粒几何形状和DNA负载之间的关系。我们发现，支化颗粒表现出增加的配体密度与纳米球，并确定积极和中性曲率可以促进额外的负载。该方法可应用于优化任何配体-核心相互作用的负载，而不依赖于纳米颗粒核心、配体或附着化学。
This paper describes a label free technique for determining ligand loading on metal nanoparticles using a variant of secondary ion mass spectrometry. Au-400(4+) clusters bombard DNA-functionalized anisotropic gold nanostars and isotropic nanospheres with similar surface areas to determine ligand density. For each projectile impact, co-localized molecules within the emission area of a single impact (diameter of 10-15 nm) were examined for each particle. Individual nanoparticle analysis allows for determination of the relationship between particle geometry and DNA loading. We found that branched particles exhibited increased ligand density versus nanospheres and determined that positive and neutral curvature could facilitate additional loading. This methodology can be applied to optimize loading for any ligand-core interaction independent of nanoparticle core, ligand, or attachment chemistry.