Network and role analysis of autophagy in Phytophthora sojae.
Network and role analysis of autophagy in Phytophthora sojae.
复制标题
大豆疫霉自噬的网络和作用分析。
DOI：
10.1038/s41598-017-01988-7
复制
发表时间：
2017-05-12
影响因子：
4.6
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Chen L;Zhang X;Wang W;Geng X;Shi Y;Na R;Dou D;Li H
自噬是真核生物中一种进化上保守的机制，在植物病原真菌的发育和致病中起重要作用。然而，卵菌中的自噬现象却鲜有报道。在这里，我们确定了26个自噬相关基因（ATGs）属于20个不同的群体在大豆疫霉使用全基因组调查，核心ATGs在卵菌被用来构建一个初步的自噬途径模型。表达谱分析显示，这些ATG广泛表达，并且其中大多数在感染阶段显著增加，表明自噬在毒力中的核心作用。在雷帕霉素和饥饿处理期间，使用GFP-PsAtg 8融合蛋白和荧光染料MDC检测大豆疫霉中的自噬。此外，自噬在孢子囊形成和孢囊萌发过程中被显著诱导。在大豆疫霉中沉默PsAtg 6a显著降低了孢子形成和致病性。此外，PsAtg 6a沉默菌株表现出吸器形成缺陷。这些结果表明，自噬在大豆疫霉的发育和侵染机制中可能起着重要作用。
Autophagy is an evolutionarily conserved mechanism in eukaryotes with roles in development and the virulence of plant fungal pathogens. However, few reports on autophagy in oomycete species have been published. Here, we identified 26 autophagy-related genes (ATGs) belonging to 20 different groups in Phytophthora sojae using a genome-wide survey, and core ATGs in oomycetes were used to construct a preliminary autophagy pathway model. Expression profile analysis revealed that these ATGs are broadly expressed and that the majority of them significantly increase during infection stages, suggesting a central role for autophagy in virulence. Autophagy in P. sojae was detected using a GFP-PsAtg8 fusion protein and the fluorescent dye MDC during rapamycin and starvation treatment. In addition, autophagy was significantly induced during sporangium formation and cyst germination. Silencing PsAtg6a in P. sojae significantly reduced sporulation and pathogenicity. Furthermore, a PsAtg6a-silenced strain showed haustorial formation defects. These results suggested that autophagy might play essential roles in both the development and infection mechanism of P. sojae.