Chromosomal deletions in Streptomyces griseus that remove the afsA locus
Chromosomal deletions in Streptomyces griseus that remove the afsA locus
复制标题
灰色链霉菌中去除 afsA 基因座的染色体缺失
DOI：
--
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
H. Kinashi
中科院分区：
文献类型：
--
作者：
A. Lezhava;D. Kameoka;H. Sugino;K. Gōshi;H. Shinkawa;O. Nimi;S. Horinouchi;T. Beppu;H. Kinashi
我们最近用限制性内切酶AseI和DraI构建了灰色链霉菌2247基因组的物理图谱，结果表明该菌株携带一条7.8Mb的线性染色体。在此基础上，构建了染色体两端的精确限制性内切酶片段和粘粒图谱，将afsA基因定位在离左端150 Kb处。两个afsA−突变体被发现遭受染色体缺失，去除了afsA基因座。缺失的大小分别为右端20和130 Kb，左端180和350 Kb。用带有缺失端点的染色体进行杂交实验表明，突变体中染色体的末端融合形成一个环状染色体。
Abstract We have recently constructed a physical map of the Streptomyces griseus 2247 genome using the restriction enzymes AseI and DraI, which revealed that this strain carries a 7.8 Mb linear chromosome. Based on this map, precise macrorestriction fragment and cosmid maps were constructed for both ends of the chromosome, which localized the afsA gene 150 Kb from the left end. Two afsA− mutants were found to have suffered chromosomal deletions that removed the afsA locus. The sizes of the deletions were 20 and 130 Kb at the right end and 180 and 350 kb at the left end, respectively. Hybridization experiments using cosmids carrying a deletion endpoint indicated that the ends of the chromosome in the mutants were fused to form a circular chromosome.