Early detection of prostate carcinogens by immunohistochemistry of HMGB2
Early detection of prostate carcinogens by immunohistochemistry of HMGB2
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DOI：
10.2131/jts.43.359
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发表时间：
2018-01-01
影响因子：
2
通讯作者：
Takahashi, Satoru
中科院分区：
文献类型：
--
作者：
Suzuki, Shugo;Kato, Hiroyuki;Takahashi, Satoru
前列腺致癌物的筛选是费时的，因为需要时间来诱导癌前病变和肿瘤病变。为了克服这一点，我们研究了替代的分子标记检测前列腺致癌物在短期内在大鼠。2-氨基-1-甲基-6-苯基咪唑[4，5-B]吡啶（PhIP）处理后，大鼠前列腺腹侧高迁移率族蛋白B2（HMGB 2）表达上调。为了评估HMGB 2在早期检测化学品致癌性的动物模型中的适用性，我们检测了大鼠前列腺中HMGB 2的表达。对6周龄雄性F344大鼠灌胃4周，共8种化学物质，根据前列腺致癌性分为两类。在研究结束时处死动物并进行HMGB 2免疫组织化学。与对照组相比，所有四种前列腺致癌物均显著增加了至少一个前列腺叶中的HMGB 2表达。相比之下，在前列腺中不致癌的四种化学物质不会导致HMGB 2上调。此外，在大鼠和人的肿瘤病变中检测到高HMGB 2表达。因此，HMGB 2的表达可能是一个很好的筛选工具，用于识别潜在的前列腺致癌物。
Screening prostatic carcinogens is time-consuming due to the time needed to induce preneoplastic and neoplastic lesions. To overcome this, we investigated alternative molecular markers for detection of prostatic carcinogens in a short period in rats. After treatment with 2-amino-l-methyl-6 -phenylimidazo[4,5-b]pyridine (PhIP), expression of high-mobility group protein B2 (HMGB2) was upregulated in rat ventral prostate. To evaluate the applicability of HMGB2 in the early detection of carcinogenicity of chemicals using animal models, we examined HMGB2 expression in prostate of rats. Six-week-old male F344 rats were gavaged for four weeks with a total of eight individual chemicals, divided into two categories based on prostate carcinogenicity. Animals were sacrificed at the end of the study and HMGB2 immunohistochemistry was performed. HMGB2 expression in least one prostate lobe was significantly increased by all four prostate carcinogens compared with the controls. In contrast, the four chemicals that were not carcinogenic in the prostate did not cause HMGB2 up-regulation. Additionally, high HMGB2 expression in neoplastic lesions in both rat and human was detected. Therefore HMGB2 expression may be a good screening tool for the identification of potential of prostate carcinogens.