Repetitive genome elements in a European corn borer, Ostrinia nubilalis, bacterial artificial chromosome library were indicated by bacterial artificial chromosome end sequencing and development of sequence tag site markers: implications for lepidopteran genomic research
Repetitive genome elements in a European corn borer, Ostrinia nubilalis, bacterial artificial chromosome library were indicated by bacterial artificial chromosome end sequencing and development of sequence tag site markers: implications for lepidopteran genomic research
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DOI：
10.1139/g08-104
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发表时间：
2009-01-01
期刊：
影响因子：
3.1
通讯作者：
Lewis, Leslie C.
中科院分区：
文献类型：
--
作者：
Coates, Brad S.;Sumerford, Douglas V.;Lewis, Leslie C.
欧洲玉米蛀虫Ostrinia nubilalis是欧洲、北美和亚洲粮食、纤维和生物燃料作物的严重害虫，也是昆虫嗅觉和物种形成的模型系统。构建了O. nubilalis包含36864个克隆，估计平均插入片段大小>= 120 kb，基因组覆盖率为8.8倍。筛选包含约2.76个基因组当量的OnB1克隆确定了24个序列标签位点标记的物理位置，包括与生态重要性和苏云金芽孢杆菌毒素抗性性状相关的标记。OnB1细菌人工染色体末端序列读段（GenBank dbGSS登录号ET 217010至ET 217273）显示与注释基因或表达序列标签的同源性，并鉴定了重复基因组元件，O. nubilalis微型亚末端反向重复转座元件（OnMITE01和OnMITE02）和ezi样长散布核元件。通过在O. nubilalis的内含子在两个不同的位点。在OnMITE01和OnMITE02的5'末端的A（GTCT）（n）四核苷酸重复是转座子介导的鳞翅目微卫星位点移动的证据。鳞翅目昆虫基因组中重复元件的数量会影响基因组的组装和标记的开发。这里描述的单基因座序列标签位点标记具有整合在连锁图谱和比较基因组研究中的下游应用。
The European corn borer, Ostrinia nubilalis, is a serious pest of food, fiber, and biofuel crops in Europe, North America, and Asia and a model system for insect olfaction and speciation. A bacterial artificial chromosome library constructed for O. nubilalis contains 36 864 clones with an estimated average insert size of >= 120 kb and genome coverage of 8.8-fold. Screening OnB1 clones comprising approximately 2.76 genome equivalents determined the physical position of 24 sequence tag site markers, including markers linked to ecologically important and Bacillus thuringiensis toxin resistance traits. OnB1 bacterial artificial chromosome end sequence reads ( GenBank dbGSS accessions ET217010 to ET217273) showed homology to annotated genes or expressed sequence tags and identified repetitive genome elements, O. nubilalis miniature subterminal inverted repeat transposable elements ( OnMITE01 and OnMITE02), and ezi-like long interspersed nuclear elements. Mobility of OnMITE01 was demonstrated by the presence or absence in O. nubilalis of introns at two different loci. A (GTCT)(n) tetranucleotide repeat at the 5' ends of OnMITE01 and OnMITE02 are evidence for transposon-mediated movement of lepidopteran microsatellite loci. The number of repetitive elements in lepidopteran genomes will affect genome assembly and marker development. Single-locus sequence tag site markers described here have downstream application for integration within linkage maps and comparative genomic studies.