lsoptericola nanjingensis sp nov., a mineral-weathering bacterium
lsoptericola nanjingensis sp nov., a mineral-weathering bacterium
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南京异孢子菌 (Isoptericola nanjingensis sp nov.)，一种矿物风化细菌
DOI：
10.1099/ijs.0.033043-0
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发表时间：
2012-04-01
影响因子：
2.8
通讯作者：
Wang, Qi
中科院分区：
文献类型：
--
作者：
Huang, Zhi;Sheng, Xia-Fang;Wang, Qi
从中国江苏省南京市的土壤中分离到一株革兰氏染色阳性、不运动、杆状或球形的放线菌，命名为H17（T）。该生物体在30 ℃、pH 7.0和3%NaCl（w/v）下生长最佳。菌株H17（T）含有L-Lys-D-Asp作为细胞壁肽聚糖类型，半乳糖、木糖和甘露糖作为全细胞糖。主要脂肪酸为anteiso-C-15：0和iso-C-15：0。总极性脂质为二磷脂酰甘油、磷脂酰甘油、磷脂、磷脂酰肌醇、磷酸糖脂、一种未鉴别糖脂和一种未鉴别脂质。甲基萘醌是MK 9（H-4）。未检出分枝菌酸。DNA G+C含量为72.4mol%。基于16 S rRNA基因序列的系统发育分析显示，该菌株与Isoptericola属的已知物种的相似性为97.8-99.6%。菌株H17（T）与等翅霉属其他种的DNA相关性较低，且具有许多区别于该属已知种的表型特征，这表明菌株H17（T）应被归类为等翅霉属的一个新种。并将该新种命名为南京等翅蛛Isoptericola nanjingensis sp. nov.。模式菌株为H17（T）（=DSM 24300（T）=CCTCC AB 2011005（T））。
A Gram-positive-staining, non-motile, rod- or coccoid-shaped actinobacterium, designated strain H17(T), was isolated from a soil sample from Nanjing, Jiangsu Province, PR China. The organism grew optimally at 30 degrees C, pH 7.0 and with 3% NaCl (w/v). Strain H17(T) contained L-Lys-D-Asp as the cell-wall peptidoglycan type and galactose, xylose and mannose as the whole-cell sugars. The major fatty acids were anteiso-C-15:0 and iso-C-15:0. The total polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylinositol, phosphoglycolipid, one unidentified glycolipid and one unidentified lipid. The menaquinone was MK9(H-4). Mycolic acids were not detected. The DNA G+C content was 72.4 mol%. Phylogenetic analysis of this strain based on 16S rRNA gene sequences revealed 97.8-99.6% similarity to recognized species of the genus lsoptericola. The low level of DNA DNA relatedness to other species of the genus lsoptericola and the many phenotypic properties that distinguished strain H17(T) from recognized species of this genus demonstrated that isolate H17(T) should be classified as representing a novel species of the genus lsoptericola. The name Isoptericola nanjingensis sp. nov. is proposed for this novel species. The type strain is H17(T) (=DSM 24300(T)=CCTCC AB 2011005(T)).