Gut microbiota can transfer fiber characteristics and lipid metabolic profiles of skeletal muscle from pigs to germ-free mice.
Gut microbiota can transfer fiber characteristics and lipid metabolic profiles of skeletal muscle from pigs to germ-free mice.
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肠道微生物群可以将猪骨骼肌的纤维特征和脂质代谢特征转移到无菌小鼠身上
DOI：
10.1038/srep31786
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发表时间：
2016-08-22
影响因子：
4.6
通讯作者：
Chen D
中科院分区：
文献类型：
--
作者：
Yan H;Diao H;Xiao Y;Li W;Yu B;He J;Yu J;Zheng P;Mao X;Luo Y;Zeng B;Wei H;Chen D
肥胖导致微生物群组成的改变，肠道微生物群的改变可以将肥胖相关的表型从供体转移到受体。肥胖荣昌猪（RP）与瘦肉约克猪（YP）相比，其肌肉纤维特征和脂质代谢谱明显不同。然而，RP是否具有与YP不同的肠道微生物群，以及微生物群与肌肉特性之间是否存在关系，目前尚不清楚。本研究旨在测试猪的肌肉特性是否可以转移到无菌（GF）小鼠。高通量焦磷酸测序证实了猪品种之间存在不同的核心微生物群，其分类分布和β多样性的调节发生了变化。RP的厚壁菌门/拟杆菌门比值显著高于YP，属差异显著。将猪微生物群移植到GF小鼠体内可以复制供体的表型。RP和GF小鼠受体表现出更高的体脂量，更高的慢收缩纤维比例，纤维大小减少，IIb纤维百分比增加，腓肠肌脂肪生成增强。此外，定植小鼠的肠道微生物群组成与其供体猪具有高度相似性。综上所述，肥胖猪的肠道微生物群本质上影响骨骼肌发育和脂质代谢谱。
Obesity causes changes in microbiota composition and an altered gut microbiota can transfer obesity-associated phenotypes from donors to recipients. Obese Rongchang pigs (RP) exhibited distinct fiber characteristics and lipid metabolic profiles in their muscle compared with lean Yorkshire pigs (YP). However, whether RP have a different gut microbiota than YP and whether there is a relationship between the microbiota and muscle properties are poorly understood. The present study was conducted to test whether the muscle properties can be transferred from pigs to germ-free (GF) mice. High-throughput pyrosequencing confirms the presence of distinct core microbiota between pig breeds, with alterations in taxonomic distribution and modulations in β diversity. RP displayed a significant higher Firmicutes/Bacteroidetes ratio and apparent genera differences compared with YP. Transplanting the porcine microbiota into GF mice replicated the phenotypes of the donors. RP and their GF mouse recipients exhibited a higher body fat mass, a higher slow-contracting fiber proportion, a decreased fiber size and fast IIb fiber percentage and enhanced lipogenesis in the gastrocnemius muscle. Furthermore, the gut microbiota composition of colonized mice shared high similarity with their donor pigs. Taken together, the gut microbiota of obese pigs intrinsically influences skeletal muscle development and the lipid metabolic profiles.