Genomic mapping of single-stranded DNA in hydroxyurea-challenged yeasts identifies origins of replication.
Genomic mapping of single-stranded DNA in hydroxyurea-challenged yeasts identifies origins of replication.
复制标题
DOI：
10.1038/ncb1358
复制
发表时间：
2006-02
影响因子：
21.3
通讯作者：
Brewer, Bonita J
中科院分区：
文献类型：
--
作者：
Feng, Wenyi;Collingwood, David;Boeck, Max E;Fox, Lindsay A;Alvino, Gina M;Fangman, Walton L;Raghuraman, Mosur K;Brewer, Bonita J
我们报告了在羟基脲存在的情况下，对野生型和检查点缺陷型rad53酵母细胞DNA复制过程中单链DNA形成的全基因组分析。在野生型细胞中，单链DNA首先出现在一部分复制起点，随后双向“迁移”，这表明单链DNA的形成与不断移动的复制叉有关。在rad53细胞中，单链DNA几乎出现在每一个已知的起点，并且随着时间的推移一直停留在那里，这表明复制叉停滞。端粒区域似乎对Rad53检查点功能的丧失特别敏感。我们还使用我们的方法绘制了粟酒裂殖酵母中的复制起点。
We report a genome-wide analysis of single-stranded DNA formation during DNA replication in wild type and checkpoint-deficient rad53 yeast cells in the presence of hydroxyurea. In wild type cells, ssDNA first appears at a subset of replication origins and later “migrates” bi-directionally, suggesting that ssDNA formation is associated with continuously moving replication forks. In rad53 cells, ssDNA appears at virtually every known origin, but remains there over time, suggesting that replication forks stall. Telomeric regions appear to be especially sensitive to the loss of Rad53 checkpoint function. We also mapped replication origins in Schizosaccharomyces pombe using our method.