Specific In Situ Visualization of the Pathogenic Endophytic Fungus Aciculosporium take, the Cause of Witches' Broom in Bamboo
Specific In Situ Visualization of the Pathogenic Endophytic Fungus Aciculosporium take, the Cause of Witches' Broom in Bamboo
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DOI：
10.1128/aem.00635-09
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发表时间：
2009-07-15
影响因子：
4.4
通讯作者：
Tanaka, Eiji
中科院分区：
文献类型：
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作者：
Tanaka, Eiji
竹类内生真菌Aciculosporium take（子囊菌门;棒孢菌科）引起竹子的连续的芽生长。定殖芽最终导致丛枝形成，但保持正常的叶排列和分枝模式。为了分析调节良好的症状发展的机制，将真菌内生菌丝在宿主组织中的位置可视化。本研究采用一种针对A.使用了take。在有或没有真菌定殖的外部迹象的病芽的组织切片上进行原位杂交。在竹组织的细胞间隙中检测到特异性信号。多数信号在茎尖分生组织和叶原基中检测到。此外，在侧芽、幼叶和茎中检测到较少的信号。这些结果表明A. take内生生长，特别是在茎顶端分生组织的主机。A的位置。采取菌丝表明，症状发展的机制可以解释的作用外源真菌生长素，连续诱导宿主内的原基启动。
The endophytic fungus Aciculosporium take (Ascomycota; Clavicipitaceae) causes continuous shoot growth in bamboo. The colonized shoot eventually results in witches' broom formation but maintains normal leaf arrangement and branching pattern. To analyze the mechanism of well-regulated symptom development, the location of the fungal endophytic hyphae in host tissues was visualized. A colorimetric in situ hybridization technique using a species-specific oligonucleotide probe targeting the 18S rRNA of A. take was used. In situ hybridization was performed on tissue sections of diseased shoots with or without external signs of fungal colonization. Specific signals were detected in intercellular spaces of the bamboo tissues. Most signals were detected in the shoot apical meristem and the leaf primordia. In addition, fewer signals were detected in the lateral buds, juvenile leaves, and stems. These results indicate that A. take grows endophytically, particularly in the shoot apical meristem of the host. The location of A. take hyphae suggests that the mechanism of symptom development can be explained by the action of exogenous fungal auxin, which continuously induces primordium initiation within the host.