Deficient Generation of Spike-Specific Long-Lived Plasma Cells in the Bone Marrow After Severe Acute Respiratory Syndrome Coronavirus 2 Infection.
Deficient Generation of Spike-Specific Long-Lived Plasma Cells in the Bone Marrow After Severe Acute Respiratory Syndrome Coronavirus 2 Infection.
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严重急性呼吸系统综合症冠状病毒 2 感染后，骨髓中尖峰特异性长寿命浆细胞生成不足。
DOI：
10.1093/infdis/jiad603
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Sajadi,MohammadM
中科院分区：
文献类型：
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作者：
Tehrani,ZahraR;Habibzadeh,Parham;Flinko,Robin;Chen,Hegang;Abbasi,Abdolrahim;Yared,JeanA;Ciupe,StancaM;Lewis,GeorgeK;Sajadi,MohammadM
稳定的长寿命浆细胞（LLPC）群体的产生是疫苗接种或感染后持久抗体反应的必要条件。我们研究了 20 名既往感染过 2019 年冠状病毒病的个体，并使用骨髓抽吸和血浆样本表征了抗体反应。我们注意到，严重急性呼吸综合征冠状病毒 2 感染后，骨髓中尖峰特异性 LLPC 的生成不足。此外，虽然回归模型解释了基于 LLPC 酶联免疫斑点测定观察到的抗破伤风免疫球蛋白 G 水平的 98% 差异，但我们无法将相同的模型与抗尖峰抗体拟合，再次表明 LLPC 对循环抗尖峰抗体缺乏贡献。
Generation of a stable long-lived plasma cell (LLPC) population is the sine qua non of durable antibody responses after vaccination or infection. We studied 20 individuals with a prior coronavirus disease 2019 infection and characterized the antibody response using bone marrow aspiration and plasma samples. We noted deficient generation of spike-specific LLPCs in the bone marrow after severe acute respiratory syndrome coronavirus 2 infection. Furthermore, while the regression model explained 98% of the observed variance in anti-tetanus immunoglobulin G levels based on LLPC enzyme-linked immunospot assay, we were unable to fit the same model with anti-spike antibodies, again pointing to the lack of LLPC contribution to circulating anti-spike antibodies.