Effects of some metabolic inhibitors on x-ray dose-response curves for the survival of mammalian cells in vitro, and on early recovery between fractionated x-ray doses.
Effects of some metabolic inhibitors on x-ray dose-response curves for the survival of mammalian cells in vitro, and on early recovery between fractionated x-ray doses.
复制标题
一些代谢抑制剂对哺乳动物细胞体外存活的 X 射线剂量反应曲线以及对分段 X 射线剂量之间早期恢复的影响。
DOI：
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发表时间：
1966
影响因子：
2.6
通讯作者：
R. Berry
中科院分区：
文献类型：
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作者：
R. Berry
通过在含有高浓度2-4-二硝基苯酚的培养基中孵育细胞，HeLa S-3 oxf细胞在体外从亚致死X射线损伤的快速（0-8小时）恢复既不延迟也不降低幅度，所述2-4-二硝基苯酚通过解偶联氧化磷酸化剥夺细胞的主要能量来源。事实上，细胞存活100 μg/ml。DNP浓度在分级X射线剂量之间显示出稍微增加的恢复。5-高浓度的氟尿嘧啶使细胞对单次X射线照射敏感，但陡峭的剂量-反应曲线保留了明显的肩部，即使药物浓度高到足以自行大幅降低细胞存活率，也没有看到两剂量恢复曲线的显著变化。抗生素嘌呤霉素和环己酰亚胺，虽然抑制蛋白质合成和敏化细胞的影响，单剂量的X射线，不减少早期恢复，即使在研究中的药物被添加到细胞培养物多达20小时前...
The rapid (0–8 hour) recovery from sub-lethal X-ray damage in HeLa S-3oxf cells in vitro is neither delayed nor reduced in magnitude by incubating the cells in media containing high concentrations of 2–4 Dinitrophenol, which deprives the cells of a major energy source by uncoupling oxidative phosphorylation. In fact, cells surviving 100 μg/ml. concentrations of DNP showed somewhat increased recovery between fractionated X-ray doses. 5-Fluorouracil in high concentrations sensitises the cells to single X-ray exposures, but the steeper dose-response curve retains a marked shoulder and no significant modification of the two-dose recovery curve is seen even with drug concentrations that are high enough to reduce cell survival drastically on their own. The antibiotics Puromycin and Cycloheximide, although inhibiting protein synthesis and sensitising cells to the effects of single doses of X rays, do not decrease early recovery even when the drug under study is added to the cell cultures as much as 20 hours prio...