Fecal Microbiome Data Distinguish Liver Recipients With Normal and Abnormal Liver Function From Healthy Controls
Fecal Microbiome Data Distinguish Liver Recipients With Normal and Abnormal Liver Function From Healthy Controls
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粪便微生物组数据可区分肝功能正常和异常的肝受体与健康对照者
DOI：
10.3389/fmicb.2019.01518
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发表时间：
2019-07-03
影响因子：
5.2
通讯作者：
Li, Lan-Juan
中科院分区：
文献类型：
--
作者：
Lu, Hai-Feng;Ren, Zhi-Gang;Li, Lan-Juan
新的证据表明，肠道微生物群的改变在许多肝脏疾病的发病机制中发挥着重要作用，主要是通过“肠道微生物群-免疫-肝脏”轴促进炎症。我们的目的是使用 16S rRNA 基因测序来研究肝功能异常/正常的肝受体的粪便微生物组。从 90 名肝脏受体 [42 名肝功能异常（LT_A 组）和 48 名肝功能正常（LT_N 组）] 和 61 名年龄和性别匹配的健康对照 (HC) 中收集粪便样本。分析粪便微生物组的微生物群落的比较组成、多样性和丰富度。主坐标分析成功区分了LT_A组受者与健康受试者的粪便微生物组，LT_A组受者粪便微生物组多样性显着下降。除了所有肝脏受体中克雷伯氏菌和埃希氏菌/志贺氏菌等机会性病原体的相对丰度较高之外，肝脏受体和 HC 之间肠道微生物组组成的主要差异是受体中产生丁酸的有益细菌的相对丰度较低。重要的是，我们建立了粪便微生物组指数（葡萄球菌和普氏菌的特异性改变），可用于区分 LT_A 组和 LT_N 组，受试者工作特征曲线下面积值为 0.801，敏感性和特异性值分别为 0.771 和 0.786。这些发现揭示了肝功能异常和正常的肝受体独特的肠道微生物特征，并确定了肝受体异常肝功能的粪便微生物风险指标。
Emerging evidence suggests that altered intestinal microbiota plays an important role in the pathogenesis of many liver diseases, mainly by promoting inflammation via the "intestinal microbiota-immunity-liver" axis. We aimed to investigate the fecal microbiome of liver recipients with abnormal/normal liver function using 16S rRNA gene sequencing. Fecal samples were collected from 90 liver recipients [42 with abnormal liver function (Group LT_A) and 48 with normal liver function (Group LT_N)] and 61 age- and gender-matched healthy controls (HCs). Fecal microbiomes were analyzed for comparative composition, diversity, and richness of microbial communities. Principal coordinates analysis successfully distinguished the fecal microbiomes of recipients in Group LT_A from healthy subjects, with the significant decrease of fecal microbiome diversity in recipients in Group LT_A. Other than a higher relative abundance of opportunistic pathogens such as Klebsiella and Escherichia/Shigella in all liver recipients, the main difference in gut microbiome composition between liver recipients and HC was the lower relative abundance of beneficial butyrate-producing bacteria in the recipients. Importantly, we established a fecal microbiome index (specific alterations in Staphylococcus and Prevotella) that could be used to distinguish Group LT_A from Group LT_N, with an area under the receiver operating characteristic curve value of 0.801 and sensitivity and specificity values of 0.771 and 0.786, respectively. These findings revealed unique gut microbial characteristics of liver recipients with abnormal and normal liver functions, and identified fecal microbial risk indicators of abnormal liver function in liver recipients.