Characterization of rotating gantry micro-CT configuration for the in vivo evaluation of murine trabecular bone.
Characterization of rotating gantry micro-CT configuration for the in vivo evaluation of murine trabecular bone.
复制标题
用于鼠小梁骨体内评估的旋转龙门微型 CT 配置的表征。
DOI：
10.1017/s1431927613001396
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hui,Susanta
中科院分区：
文献类型：
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作者：
Arentsen,Luke;Hui,Susanta
本研究的目的是确定最佳的物理参数的旋转机架micro-CT系统，以评估在体内变化的小鼠骨小梁。在市售微型CT系统上检查放大率、分箱、峰值千伏、射束过滤和组织厚度。X射线源和探测器几何结构提供1.3×、1.8×或3.3×放大倍数。从无分箱到2到4检查分箱。能量以10 kVp的增量从40 kVp变化到80 kVp，过滤器厚度以0.5 mmAl的增量从无过滤增加到1.5 mmAl。以不同的放大率和分箱组合对小鼠成像，以评价图像质量和微结构估计的变化。将放大倍率从1.3倍增加到3.3倍，并将面元从4降低到1，空间分辨率从2.5 lp/mm变化到11.8 lp/mm。增加光束能量或过滤厚度会降低亨氏单位（HU）估计值，最大变化率为−286 HU/kVp（80 kVp）。有效像素尺寸大于60 μm时，小鼠骨小梁的图像会变得模糊。通过比较分辨率、信噪比和辐射剂量，我们发现，3.3倍放大率、2.80 kVp光束与0.5 mmAl滤波器的合并是评价该旋转机架micro-CT小鼠骨小梁的最佳参数。
The objective of this study is to determine the optimal physical parameters of a rotating gantry micro-CT system to assessin vivochanges to the trabecular bone of mice. Magnification, binning, peak kilovoltage, beam filtration, and tissue thickness are examined on a commercially available micro-CT system. The X-ray source and detector geometry provides 1.3×, 1.8×, or 3.3× magnification. Binning is examined from no binning to 2 to 4. Energy is varied from 40 to 80 kVp in 10 kVp increments and filter thickness is increased from no filtration to 1.5 mmAl in 0.5 mmAl increments. Mice are imaged at different magnifications and binning combinations to evaluate changes to image quality and microstructure estimation. Increasing magnification from 1.3× to 3.3× and lowering binning from 4 to 1 varies the spatial resolution from 2.5 to 11.8 lp/mm. Increasing the beam energy or filtration thickness decreases Hounsfield unit (HU) estimation, with a maximum rate of change being −286 HU/kVp for 80 kVp. Images for murine trabecular bone are blurred at effective pixel sizes above 60 μm. By comparing resolution, signal-to-noise ratio, and radiation dose, we find that a 3.3× magnification, binning of 2.80 kVp beam with a 0.5 mmAl filter comprises the optimal parameters to evaluate murine trabecular bone for this rotating gantry micro-CT.