Comparative study of gene expression and major proteins' function of laticifers in lignified and unlignified organs of mulberry
Comparative study of gene expression and major proteins' function of laticifers in lignified and unlignified organs of mulberry
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DOI：
10.1007/s00425-011-1533-6
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发表时间：
2012-03-01
期刊：
影响因子：
4.3
通讯作者：
Hori, Yusuke
中科院分区：
文献类型：
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作者：
Kitajima, Sakihito;Taira, Toki;Hori, Yusuke
乳管是一种参与植物防御生物胁迫（如食草动物和微生物）的细胞;然而，对其基因表达知之甚少。我们比较了蛋白质的积累和转录组之间的乳汁管的木质部和unligniferous器官的桑树（桑树），它有一个非关节，分支类型的乳汁管。LA-a（相当于MLX 56）及其同源物LA-b（含有两个几丁质结合结构域的杀虫几丁质酶样蛋白）是非木质器官乳管中的主要蛋白，另一种蛋白（LA-c）是木质器官乳管中的主要蛋白。纯化、cDNA克隆和生物活性测定结果表明，LA-c是一种酸性Ⅰ类几丁质酶，具有抗真菌活性，但不具有杀虫活性。使用GS-FLX的比较mRNA-Seq分析揭示了其他可能的防御相关蛋白的转录本。此外，花豆素样凝集素、半乳糖醛酸酶抑制剂和病程相关蛋白也很丰富，但其相对含量在木质部和非木质部乳管中存在差异。结果表明，植物乳管网络是不连续的，这些器官对不同的潜在敌人的适应。
A laticifer is a cell involved in plant defense against biotic stresses such as herbivores and microorganisms; however, its gene expression is poorly understood. We compared protein accumulation and transcriptomes among laticifers of lignified and unlignified organs of mulberry (Morus alba), which has a non-articulated, branched type of laticifer. LA-a (equivalent to MLX56) and its homolog LA-b (insecticidal chitinase-like proteins containing two chitin-binding domains) were major proteins in laticifers of unlignified organs, and another protein (LA-c) was a major protein in laticifers of lignified organs. Purification, cDNA cloning, and bioassay of LA-c revealed that LA-c was an acidic class I chitinase having antifungal but not insecticidal activity. Comparative mRNA-Seq analysis using a GS-FLX revealed transcripts of other possible defense-related proteins. Jacalin-like lectin, galacturonase-inhibitor, and pathogenesis-related proteins were also abundant; however, the relative amounts differed among laticifers of lignified and unlignified organs. The results suggest a discontinuous laticifer network in planta and adaptation to different potential enemies among these organs.