Detection of human leptin in serum using chemiluminescence immunosensor: Signal amplification by hemin/G-quadruplex DNAzymes and protein carriers by Fe3O4/polydopamine/Au nanocomposites
Detection of human leptin in serum using chemiluminescence immunosensor: Signal amplification by hemin/G-quadruplex DNAzymes and protein carriers by Fe3O4/polydopamine/Au nanocomposites
复制标题
使用化学发光免疫传感器检测血清中的人瘦素：氯化血红素/G-四链体 DNA 酶和 Fe3O4/聚多巴胺/Au 纳米复合材料的蛋白质载体放大信号
DOI：
10.1016/j.snb.2015.07.022
复制
发表时间：
2015-12-31
影响因子：
8.4
通讯作者：
Wang, Lun
中科院分区：
文献类型：
--
作者：
He, Yuezhen;Sun, Jian;Wang, Lun
利用催化氯化高铁血红素/G-四链脱氧核糖核酸酶和功能性超顺磁性纳米复合材料的多重信号放大策略，研制了一种用于灵敏检测人瘦素的夹心式化学发光免疫传感器。为了构建该传感平台，以聚多巴胺(Pd)为粘结剂，采用原位法制备了核壳结构的Fe3O4/Pd/Au超顺磁性纳米复合材料。用扫描电子显微镜、透射电子显微镜、能量色散光谱、傅里叶变换红外光谱和室温振动样品磁强计对所制备的纳米复合材料进行了进一步表征。在该免疫传感器中，抗人瘦素的单抗(捕获抗体)被结合到Fe3O4/Pd/Au纳米复合材料上。人瘦素、生物素标记的检测抗体和链霉亲和素-DNA酶通过特定的相互作用先后与上述纳米复合材料结合形成夹心型免疫复合体。负载免疫复合物的磁性粒子被外加磁铁分离，免疫复合物中的DNA酶大大增强了鲁米诺-H_2O_2体系的化学发光。该免疫传感器对人瘦素的检测具有较高的灵敏度、良好的特异性和较宽的线性范围，检测范围为1.0~8.0×10~(2)pg·L~(-1)，最低检测下限为0.3 pg·L~(-1)。该传感器是检测瘦素最灵敏的方法之一，因为DNAzyme的高效催化作用和分析物对磁性捕获的富集性。(C)2015爱思唯尔B.V.保留所有权利。
A sandwich chemiluminescence (CL) immunosensor for sensitive detection of human leptin was developed with a multiple signal amplification strategy from catalytic hemin/G-quadruplex DNAzymes and functional superparamagnetic nanocomposites. To construct this sensing platform, core-shell structural Fe3O4/polydopamine (PD)/Au superparamagnetic nanocomposites were synthesized by an in situ method with PD as a substrate adhesive. These nanocomposites were further characterized by scanning electron microscopy, transmission electronic microscopy, energy-dispersive spectroscopy, Fourier transform infrared spectrum and a vibrating sample magnetometer at room temperature. In this immunosensor, the monoclonal anti-human leptin antibody (capture antibody) was bound to the Fe3O4/PD/Au nanocomposites. Human leptin, biotinylated detection antibodies and streptavidin-DNAzymes were successively combined the above-mentioned nanocomposites to form sandwich-type immunocomplex through specific interactions. The magnetic particles loaded with the immunocomplex were separated by an external magnet, and the DNAzymes in the immunocomplex greatly enhanced the CL emission of the luminol-H2O2 system. The immunosensor exhibited a high sensitivity, a good specificity, and a wide linear range for human leptin detection from 1.0 pg mL(-1) to 8.0 x 10(2) pg mL(-1) with a low detection limit of 0.3 pg mL(-1). This sensor is one of the most sensitive methods for leptin detection due to the highly efficient catalysis of the DNAzymes and analyte enrichment on magnetic capture. (C) 2015 Elsevier B.V. All rights reserved.