THE SPECIFIC VELOCITY PLOT - A GRAPHICAL-METHOD FOR DETERMINING INHIBITION PARAMETERS FOR BOTH LINEAR AND HYPERBOLIC ENZYME-INHIBITORS
THE SPECIFIC VELOCITY PLOT - A GRAPHICAL-METHOD FOR DETERMINING INHIBITION PARAMETERS FOR BOTH LINEAR AND HYPERBOLIC ENZYME-INHIBITORS
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DOI：
10.1111/j.1432-1033.1981.tb05570.x
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
BAICI, A
中科院分区：
文献类型：
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作者：
BAICI, A
描述了一种新的方法，用于绘制受抑制的酶促反应的动力学结果[通过糖胺聚糖多硫酸盐的人白细胞弹性蛋白酶（EC 3.4.21.37），通过G-6-P的粗糙脉孢菌糖原磷酸化酶（EC 2.4.1.1）]。该图特别设计用于分析双曲线、混合型抑制剂，但也可应用于任何其他线性系统。该方法包括以标准化的方式绘制实验数据，如vo/vi对比速度σ/v。（1 + δ），其中vo/vi表示在给定底物浓度[S]下非抑制反应和抑制反应的初始速度之比，并且σ是[S]/Km比值。该程序提供了一种确定抑制常数Ki和K“i的简单方法，即，EI和ESI络合物的解离常数，分别，和速度饱和值。
A new method is described for plotting kinetic results for inhibited enzymatic reactions [human leukocyte elastase (EC 3.4.21.37) by a glycosaminoglycan polysulfate, Neurospora crassa glycogen phosphorylase (EC 2.4.1.1) by G-6-P]. The plot is particularly designed for the analysis of hyperbolic, mixed-type inhibitors, but can be applied to any other linear system. The method consists of plotting experimental data in a normalized way, as vo/vi versus the specific velocity .sigma./(1 + .delta.), where vo/vi represents the ratio of the initial velocities for the non-inhibited and inhibited reactions at a given substrate concentrations [S], and .sigma. is the [S]/Km ratio. The procedure provides a simple way of determining the inhibition constants Ki and K''i, i.e., the dissociation constants of the EI and ESI complexes, respectively, and the velocity saturation values.