Expression of MMP-8 and MMP-13 genes in the periodontal ligament during tooth movement in rats
Expression of MMP-8 and MMP-13 genes in the periodontal ligament during tooth movement in rats
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DOI：
10.1177/154405910308200815
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发表时间：
2003-08-01
影响因子：
7.6
通讯作者：
Mitani, H
中科院分区：
文献类型：
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作者：
Takahashi, I;Nishimura, M;Mitani, H
在正畸牙齿移动过程中，牙周韧带组织在移动牙齿的张力侧和压力侧都被重塑。本研究旨在阐明正畸牙移动过程中牙周韧带组织改建过程中，基质金属蛋白酶-8和基质金属蛋白酶-13mRNA表达增强的假说。采用原位杂交法和半定量逆转录/聚合酶链式反应分析方法，研究基质金属蛋白酶-8和基质金属蛋白酶-13的基因表达。在活体牙齿移动过程中，受压侧和张力侧的基质金属蛋白酶-8和基质金属蛋白酶-13mRNA的表达均有一过性增加。在体外，拉伸可诱导基质金属蛋白酶-8和基质金属蛋白酶-13的基因表达，而压缩通过可溶性因子间接促进基质金属蛋白酶-8和基质金属蛋白酶-13的基因表达。因此，我们认为，基质金属蛋白酶-8和基质金属蛋白酶-13的表达受拉伸和压缩的不同调节，在牙周膜重塑过程中起重要作用。
Periodontal ligament tissue is remodeled on both the tension and compression sides of moving teeth during orthodontic tooth movement. The present study was designed to clarify the hypothesis that the expression of MMP-8 and MMP-13 mRNA is promoted during the remodeling of periodontal ligament tissue in orthodontic tooth movement. We used the in situ hybridization method and semi-quantitative reverse-transcription/polymerase chain-reaction analysis to elucidate the gene expression of MMP-8 and MMP-13 mRNA. Expression of MMP-8 and MMP-13 mRNA transiently increased on both the compression and tension sides during active tooth movement in vivo. The gene expression of MMP-8 and MMP-13 was induced by tension, while compression indirectly promoted the gene expression of MMP-8 and MMP-13 through soluble factors in vitro. Thus, we concluded that the expression of MMP-8 and MMP-13 is differentially regulated by tension and compression, and plays an important role in the remodeling of the periodontal ligament.