Myosin II-independent processes in mitotic cells of Dictyostelium discoideum: redistribution of the nuclei, re-arrangement of the actin system and formation of the cleavage furrow.
Myosin II-independent processes in mitotic cells of Dictyostelium discoideum: redistribution of the nuclei, re-arrangement of the actin system and formation of the cleavage furrow.
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盘基网柄菌有丝分裂细胞中不依赖肌球蛋白 II 的过程：细胞核的重新分布、肌动蛋白系统的重新排列以及卵裂沟的形成。
DOI：
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发表时间：
1997
影响因子：
4
通讯作者：
Günther Gerisch
中科院分区：
文献类型：
--
作者：
R. Neujahr;C. Heizer;Günther Gerisch
研究了缺乏肌球蛋白II的盘基网柄菌多核和单核突变细胞的有丝分裂(Manstein等人。(1989)EMBO J.8,923-932)。多核细胞是通过悬浮培养产生的，单核细胞通过在固体表面生长而丰富(DeLozanne和Spudich(1987)Science 236,1086-1091)。多核细胞揭示了有丝分裂复合体与细胞皮质的相互作用，这在正常的单核细胞中并不明显。在后期，由纺锤体、微管和分离的染色体组成的整个有丝分裂复合体被转移到细胞的外围。这些复合体被附加在距离细胞表面约3微米的地方，以一种纺锤形变得平行于细胞表面的方式。随后，在微管的顶端周围形成细胞表面的裂片。这些脑叶上覆盖着大量的锥形突起，这是一种肌动蛋白相关蛋白，通常在动态的细胞表面投射中积累(DeHostos等人)。(1991)EMBO J.10,4097-4104)。在固体表面的生长过程中，单核肌球蛋白II缺失的细胞经历了有丝分裂分裂阶段，其速度与野生型细胞相当。与有丝分裂相关的胞质分裂与牵引介导的细胞分裂有几个参数的区别，后者导致间期多核细胞的碎片被掐断。讨论了细胞在有丝分裂过程中分裂的可能性，而不是在分裂沟的位置形成收缩环。
Mitosis was studied in multinucleated and mononucleated mutant cells of Dictyostelium discoideum that lack myosin II (Manstein et al. (1989) EMBO J. 8, 923-932). Multinucleated cells were produced by culture in suspension, mononucleated cells were enriched by growth on a solid surface (DeLozanne and Spudich (1987) Science 236, 1086-1091). The multinucleated cells disclosed interactions of mitotic complexes with the cell cortex that were not apparent in normal, mononucleated cells. During the anaphase stage, entire mitotic complexes consisting of spindle, microtubule asters, and separated sets of chromosomes were translocated to the periphery of the cells. These complexes were appended at a distance of about 3 microns from the cell surface, in a way that the spindle became orientated in parallel to the surface. Subsequently, lobes of the cell surface were formed around the asters of microtubules. These lobes were covered with tapered protrusions rich in coronin, an actin associated protein that typically accumulates in dynamic cell-surface projections (DeHostos et al. (1991) EMBO J. 10, 4097-4104). During their growth on a solid surface, mononucleated myosin II-null cells passed through the mitotic cleavage stages with a speed comparable to wild-type cells. Cytokinesis as linked to mitosis is distinguishable by several parameters from traction mediated cytofission, which results in the pinching off of pieces of a multinucleated cell in the interphase. The possibility is discussed that cells can cleave during mitosis without forming a contractile ring at the site of the cleavage furrow.
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影响因子：
--
作者：
FUKUI, Y;INOUE, S
通讯作者：
INOUE, S
影响因子：
2.7
作者：
WESSELS, D;SOLL, DR;SPUDICH, J
通讯作者：
SPUDICH, J
影响因子：
11.4
作者：
MANSTEIN, DJ;TITUS, MA;SPUDICH, JA
通讯作者：
SPUDICH, JA
肌球蛋白-抗体复合物对上皮样细胞收缩环和圆周带的不同影响。
影响因子：
4
作者：
Zurek,B;Sanger,JM;Sanger,JW;Jockusch,BM
通讯作者：
Jockusch,BM
影响因子：
56.9
作者：
DELOZANNE, A;SPUDICH, JA
通讯作者：
SPUDICH, JA