Immortalization of human myogenic progenitor cell clone retaining multipotentiality
Immortalization of human myogenic progenitor cell clone retaining multipotentiality
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DOI：
10.1016/j.bbrc.2006.08.006
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发表时间：
2006-10-06
影响因子：
3.1
通讯作者：
Inagawa, Masayo
中科院分区：
文献类型：
--
作者：
Hashimoto, Naohiro;Kiyono, Tohru;Inagawa, Masayo
人肌原细胞在培养中增殖能力有限。虽然端粒酶的强制表达可以使某些细胞类型永生化，但端粒酶单独延缓了原代培养的人肌原细胞的衰老，但不能使其永生化。相比之下，端粒酶和E7基因从人乳头瘤病毒16型的组成型表达永生原代人肌细胞。我们通过异位表达端粒酶和E7，建立了一个永生化的原代人肌源性细胞系。永生化的人肌源性细胞表现出其主要亲本的表型特征，包括在适当的培养条件下经历肌源性、成骨性和成脂性终末分化的能力。永生化细胞将用于针对人类肌肉疾病的基础和应用研究。此外，通过转导端粒酶和E7的永生化代表了一种有用的方法，通过该方法在体外扩增人肌源性细胞而不损害其分化能力。(c)2006爱思唯尔公司All rights reserved.
Human myogenic cells have limited ability to proliferate in culture. Although forced expression of telomerase can immortalize some cell types, telomerase alone delays senescence of human primary cultured myogenic cells, but fails to immortalize them. In contrast, constitutive expression of both telomerase and the E7 gene from human papillomavirus type 16 immortalizes primary human myogenic cells. We have established an immortalized primary human myogenic cell line preserving multipotentiality by ectopic expression of telomerase and E7. The immortalized human myogenic cells exhibit the phenotypic characteristics of their primary parent, including an ability to undergo myogenic, osteogenic, and adipogenic terminal differentiation under appropriate culture conditions. The immortalized cells will be useful for both basic and applied studies aimed at human muscle disorders. Furthermore, immortalization by transduction of telomerase and E7 represents a useful method by which to expand human myogenic cells in vitro without compromising their ability to differentiate. (c) 2006 Elsevier Inc. All rights reserved.