A guide to accurate reporting in digital image acquisition - can anyone replicate your microscopy data?
A guide to accurate reporting in digital image acquisition - can anyone replicate your microscopy data?
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DOI：
10.1242/jcs.254144
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发表时间：
2021-03-30
影响因子：
4
通讯作者：
Chew, Teng-Leong
中科院分区：
文献类型：
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作者：
Heddleston, John M;Aaron, Jesse S;Chew, Teng-Leong
最近的技术进步使显微镜在生命科学研究中不可或缺。它的无处不在的使用，反过来，强调了确保基于显微镜的实验是可复制的，以及结果数据具有可比性的重要性。虽然已经有大量的综述文章、实用指南和会议致力于维护标准仪器操作条件的主题，但不可否认的是，致力于正确记录显微镜实验的关注很少。缺乏对准确报道的重视不仅限于生命科学研究人员本身，还延伸到许多期刊的审查小组和编辑委员会。不幸的是，在传达科学发现的最后一步，这种疏忽可能会否定许多以科学重现性的名义确保实验质量控制的英勇努力。这篇综述旨在列举在成像实验中应该报告的各种参数，说明它们的不一致应用如何导致不可调和的结果。
Recent technological advances have made microscopy indispensable in life science research. Its ubiquitous use, in turn, underscores the importance of ensuring that microscopy-based experiments are replicable and that the resulting data comparable. While there has been a wealth of review articles, practical guides and conferences devoted to the topic of maintaining standard instrument operating conditions, the paucity of attention dedicated to properly documenting microscopy experiments is undeniable. This lack of emphasis on accurate reporting extends beyond life science researchers themselves, to the review panels and editorial boards of many journals. Such oversight at the final step of communicating a scientific discovery can unfortunately negate the many valiant efforts made to ensure experimental quality control in the name of scientific reproducibility. This Review aims to enumerate the various parameters that should be reported in an imaging experiment by illustrating how their inconsistent application can lead to irreconcilable results.