Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse.
Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse.
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三斑濑鱼成体睾丸中 doublesex-mab 3 相关转录因子 1 (DMRT1) 定位的组织学观察。
DOI：
10.1007/s40071-014-0068-4
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发表时间：
2014
期刊：
影响因子：
1.3
通讯作者：
M Nakamura
中科院分区：
文献类型：
--
作者：
Kobayashi Y;R Nozu;R Horiguchi;M Nakamura
已确定Doublemex-mab 3相关转录因子1（DMRT 1）是参与脊椎动物睾丸分化的第一个保守基因。然而，DMRT 1在精子发生中的确切作用尚不清楚。本研究采用免疫组织化学方法观察DMRT 1蛋白在三点隆头鱼精巢中的定位，以解析DMRT 1在精巢中的细胞分布。DMRT 1蛋白明确和具体定位于支持细胞的所有生精细胞和上皮细胞组成的输出管，但不是在生殖细胞。此外，成年男性用芳香化酶抑制剂（AI）处理，以研究雌激素对DMRT 1转录的作用。AI治疗引起的输出管区域的DMRT 1转录水平的增加，伴随着精原细胞和精母细胞的减少。
Doublesex-mab 3-related transcription factor 1 (DMRT1) has been identified as the first conserved gene involved in the testicular differentiation of vertebrates. However, the precise role of DMRT1 in spermatogenesis has not been made clear. In this study, immunohistochemical method was used to observe DMRT1 protein localization in order to resolve cellular profile of DMRT1 in the adult testis of three-spot wrasse. DMRT1 protein was clearly and specifically localized in the Sertoli cells of all spermatogenic cells and epithelial cells comprising the efferent duct, but not in the germ cells. In addition, adult males were treated with aromatase inhibitor (AI) for investigating the role of estrogen on the transcription of DMRT1. AI treatment caused an increase in the levels of DMRT1 transcripts in the efferent duct region, concomitant with a decrease in spermatogonia and spermatocytes.