Reentrant condensation of a multicomponent complex system of biomolecules induced by polyphosphate-猫眼课题宝

DOI：

10.1101/2023.03.02.530750

发表时间：

2023-03

期刊：

bioRxiv

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

T. Furuki;Tomohiro Nobeyama;Shunji Suetaka;Ryokei Matsui;Tatsuhiko Fukuoka;M. Arai;K. Shiraki

再入缩合（RC）是蛋白质溶液的一种相行为，至少包括两种组分。在RC中，随着一种组分的浓度单调增加，蛋白质状态从一个相变为两个相，然后回到一个相。为了理解生物分子多组分复杂溶液的相行为，有必要构建一个具有RC行为的实验多组分体系。在这里，我们使用了可乐/牛奶混合物，调查RC的多组分复杂系统，并解释RC机制，通过减少系统的两个纯组分，聚磷酸盐（聚P）和酪蛋白。在多组分复合体系中，20-60%可乐和1%牛奶可观察到RC。在纯系统中，RC发生0.01-2 mM四磷酸和0.5 mg/ml酪蛋白。此外，相图显示酪蛋白的缩合取决于聚磷的链长。本研究成功地在实验上诱导RC在多组分系统和再现RC，即使当系统减少到其纯组分。使用普通材料可以实验诱导RC的事实将为理解生物分子的相分离行为提供重要的见解。

Reentrant condensation (RC) is a phase behavior of protein solution comprising at least two components. In RC, a protein state varies from one phase to two phases and then back to one phase as the concentration of one component monotonically increases. To understand the phase behavior of multicomponent complex solutions of biomolecules, it is worth constructing an experimental multicomponent system that exhibits RC behavior. Here, we used a cola/milk mixture to investigate RC of a multicomponent complex system and explained the RC mechanism by reducing the system to two pure components, polyphosphate (polyP) and casein. In the multicomponent complex system, RC was observed with 20–60% cola and 1% milk. In the pure system, RC occurred with 0.01–2 mM tetraphosphate and 0.5 mg/ml casein. Moreover, the phase diagram showed that the condensation of casein depended on the chain length of the polyP. The present study succeeded in experimentally inducing RC in a multicomponent system and reproducing RC even when the system was reduced to its pure components. The fact that RC can be experimentally induced using common materials will provide important insights into the understanding of phase-separation behavior of biomolecules.

Reentrant condensation of a multicomponent complex system of biomolecules induced by polyphosphate

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