Variation in “bank of microscopic forms”in urchin barren coast : detection using DNA metabarcoding based on high-throughput sequencing
Variation in “bank of microscopic forms”in urchin barren coast : detection using DNA metabarcoding based on high-throughput sequencing
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海胆贫瘠海岸“微观形态库”的变异：基于高通量测序的DNA元条形码检测
DOI：
10.1007/s10811-020-02122-3
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发表时间：
2020
影响因子：
3.3
通讯作者：
Satoshi Nagai
中科院分区：
文献类型：
--
作者：
Shingo Akita;Hiroki Murasawa;Yoshihito Takano;Yoko Kawakami;Daisuke Fujita;Satoshi Nagai
从海胆贫瘠地恢复的大型藻床的类型是由“微观形态银行”的组成部分决定的。在本研究中，我们研究了海胆荒地（UB1、UB2、UB5和UB7）的四种不同植被和深度（1、2、5和7 m）以及不同生态阶段（EB、海带床和SC、软珊瑚床）的卵石上的河岸成分的变化，特别是藻门和红藻门。我们从6个站点收集了30个鹅卵石（每个站点3-7个鹅卵石），并基于高通量测序的DNA元条形码对附着的藻类进行了鉴定。在筛选的数据中，共检测到74个大型藻类操作分类单元（otu），其中Phaeophyceae和Rhodophyta分别为32个和42个。海胆荒漠OTU丰富度随深度增加，从UB1（0.07±0.03 OTUs cm - 2）增加到UB7(0.21±0.12 OTUs cm - 2)，与其他生态阶段（EB和SC）相似。UB7的Shannon-Weiner多样性指数也显著最高，与EB和SC相似（p< 0.05）。然而，排序测试（非度量多维标度）将EB划分为除了UB7和SC之外的不同组，可能是由于EB仅以Phaeophyceae为主。因此，大藻丰富度和组成可分别由深度和生态阶段决定。此外，在收集的鹅卵石UB7上检测到OTU鉴定的裙带菜。因此，在海胆贫瘠的海岸，海带配子体的丰富度也可能越深越高。
Type of macroalgal beds that recovered from sea urchin barrens is determined by components of “bank of microscopic forms.” In the present study, we investigated the variation of the bank components, especially in the Phaeophyceae and Rhodophyta, on cobbles at four different vegetation and depths (1, 2, 5, and 7 m) of urchin barren grounds (UB1, UB2, UB5, and UB7), and alternative ecological phases (EB, ecklonian kelp bed and SC, soft coral bed). We collected 30 cobbles (3–7 cobbles per station) from the six stations and identified the attached algae by DNA metabarcoding based on high-throughput sequencing. In the rarefied data, 74 macroalgal operational taxonomic units (OTUs) were detected, of which 32 and 42 OTUs were assigned to Phaeophyceae and Rhodophyta, respectively. The OTU richness in urchin barrens increased with depth from UB1 (0.07 ± 0.03 OTUs cm−2of cobble) to UB7 (0.21 ± 0.12 OTUs cm−2of cobble), which was similar to the other ecological phases (EB and SC). The Shannon-Weiner diversity index in UB7 was also significantly highest and similar to EB and SC (p< 0.05). However, the ordination test (non-metric multidimensional scaling) assigned EB to different group apart from UB7 and SC, probably due to solely Phaeophyceae dominance in EB. Therefore, macroalgal richness and components of the bank could be determined by depth and ecological phases, respectively. Furthermore, OTU identified asUndaria pinnatifidawas detected on a cobble collected UB7. Thus, in urchin barren coast, the richness in kelp gametophyte is probably also higher in deeper area.