Research Articles : Microsatellite DNA Somaclonal Variation of Regenerated Plants via Cotyledon Culture of Hot Pepper (Capsicum annuum L.)
Research Articles : Microsatellite DNA Somaclonal Variation of Regenerated Plants via Cotyledon Culture of Hot Pepper (Capsicum annuum L.)
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发表时间：
2007
期刊：
影响因子：
2.1
通讯作者：
T. Ryu;S. Yi;Yong-Sham Kwon;Byung-Dong Kim
中科院分区：
文献类型：
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作者：
T. Ryu;S. Yi;Yong-Sham Kwon;Byung-Dong Kim
本研究利用微卫星DNA标记对辣椒子叶培养再生植株的体细胞无性系变异进行了研究。苏比乔变种。利用25对微卫星引物对41个子叶再生植株进行了筛选。在两个SSR位点上检测到再生植株的微卫星DNA变异。SSR标记HpmsF15和HpmsF037在41株试管苗中有5株发生了体细胞无性系变异，变异率为12%。然而，23个微卫星标记证明没有核DNA多态性。然而，微卫星标记技术可以成功地用于评估辣椒体细胞无性系变异的遗传变异。
In this study, microsatellite DNA markers were utilized to characterize somaclonal variation in regenerated plants from cultured cotyledons of Capsicum annuum L. cv Subicho. Twenty five microsatellite primer pairs were utilized in the screening of 41 regenerated plants derived from the cotyledons. Microsatellite DNA variation was detected among the regenerated plants at two SSR loci. The primer pairs for the SSR loci HpmsF15 and HpmsF037 revealed somaclonal variation in 5 out of the 41 plantlets (12%). However, 23 microsatellite markers evidenced no nuclear DNA polymorphisms. Nevertheless, the microsatellite marker technique can be successfully employed to assess genetic variations in somaclonal variants of the hot pepper.