Transcription factor sumoylation and factor YY1 serve to modulate mouse amelogenin gene expression.
Transcription factor sumoylation and factor YY1 serve to modulate mouse amelogenin gene expression.
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转录因子 sumoylation 和因子 YY1 用于调节小鼠牙釉蛋白基因表达。
DOI：
10.1111/j.1600-0722.2006.00319.x
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Snead,MalcolmL
中科院分区：
文献类型：
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作者：
Xu,Yucheng;Zhou,YanL;Ann,DavidK;MacDougald,OrmondA;Shum,Lillian;Snead,MalcolmL
釉原蛋白在控制釉质生物矿化中是必不可少的，因此釉原蛋白基因受到时空调控以确保适当的釉原蛋白表达。在这项研究中，我们研究了sumoylation改变CCAAT/增强子结合蛋白α（C/EBPα）活性的作用，并使用蛋白质/DNA阵列系统搜索与C/EBPα协同作用改变釉原蛋白表达的其他蛋白质。我们观察到C/EBPα被SUMO化修饰，这种修饰对C/EBPα活性的调节起间接抑制作用，而C/EBPα活性似乎是通过其他转录因子起作用的。蛋白质/DNA阵列使我们能够挑出转录因子YY 1，它在没有直接DNA结合的情况下抑制基础釉原蛋白启动子活性和C/EBPα介导的转录激活。两者合计，这些途径可能占牙釉原蛋白基因表达的生理调节的一部分，根据牙齿发育和釉质生物矿化的要求。
Amelogenin proteins are essential in the control of enamel biomineralization and the amelogenin gene therefore is spatiotemporally regulated to ensure proper amelogenin protein expression. In this study, we examined the role of sumoylation to alter CCAAT/enhancer‐binding protein alpha (C/EBPα) activity, and performed a search using a protein/DNA array system for other proteins that act co‐operatively with C/EBPαto alter amelogenin expression. We observed that C/EBPαwas modified by sumoylation, and that this modification played an indirect inhibitory role on the regulation of C/EBPαactivity which appeared to act through other transcription factors. The protein/DNA array allowed us to single out the transcription factor, YY1, which acts in the absence of direct DNA binding to repress both the basal amelogenin promoter activity and C/EBPα‐mediatedtransactivation. Taken together, these pathways may account for part of the physiological modulation of the amelogenin gene expression in accordance with tooth developmental and enamel biomineralization requirements.