ANTIGENIC DIVERSITY OF LIPOOLIGOSACCHARIDES OF NONTYPABLE HAEMOPHILUS-INFLUENZAE
ANTIGENIC DIVERSITY OF LIPOOLIGOSACCHARIDES OF NONTYPABLE HAEMOPHILUS-INFLUENZAE
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DOI：
10.1128/iai.55.4.882-887.1987
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发表时间：
1987-04-01
影响因子：
3.1
通讯作者：
APICELLA, MA
中科院分区：
文献类型：
--
作者：
CAMPAGNARI, AA;GUPTA, MR;APICELLA, MA
非典型流感嗜血杆菌的脂寡糖（LOS）是一组抗原性异质的大分子。免疫扩散和酶联免疫吸附试验抑制研究与苯酚-水提取的LOS和吸收的抗血清特异性的寡糖部分的LOS确定了六个LOS菌株特异性抗原。为了便于筛选大量菌株以寻找LOS抗原异质性，开发了在Western印迹中利用蛋白酶K全细胞酶的系统。72个不典型的H。在该蛋白质印迹分析中分析流感病毒LOS提取物。根据一种或多种兔抗血清的免疫识别，这些提取物中的37种可被分离成10个抗原性不同的LOS组。35株不含这些LOS抗原。这些结果表明，抗原性差异之间存在的LOS的不可分型H。流感病毒株，这种异质性有可能被用来建立一个基于LOS的血清分型系统。
The lipooligosaccharides (LOS) of nontypable Haemophilus influenzae are an antigenically heterogeneous group of macromolecules. Immunodiffusion and enzyme-linked immunosorbent assay inhibition studies with phenol-water-extracted LOS and absorbed antisera specific for the oligosaccharide portion of the LOS identified six LOS strain-specific antigens. To facilitate screening large numbers of strains to search for LOS antigenic heterogeneity, a system utilizing proteinase K whole cell digests in Western blots was developed. Seventy-two nontypable H. influenzae LOS extracts were analyzed in this Western blot assay. Thirty-seven of these extracts could be segregated into 10 antigenically distinct LOS groups based on immunologic recognition by one or more of the rabbit antisera. Thirty-five of the strains did not contain these LOS antigens. These results demonstrate that antigenic differences exist among the LOS of nontypable H. influenzae strains, and this heterogeneity has the potential to be used to establish an LOS-based serogrouping system.