Tumor-localizing and photosensitizing properties of the main components of hematoporphyrin derivative.
Tumor-localizing and photosensitizing properties of the main components of hematoporphyrin derivative.
复制标题
血卟啉衍生物主要成分的肿瘤定位和光敏特性。
作者：
J. Evensen;S. Sommer;J. Moan;T. Christensen
采用高压液相色谱法和凝胶渗透色谱法对3 H标记和未标记的血卟啉衍生物（HPD）进行分析。采用高效液相色谱法分离得到四个主要成分，凝胶渗透色谱法分离得到两个主要成分。用刘易斯肺癌荷瘤小鼠体内注射法测定各组分的肿瘤定位能力，并与~（67）Ga和~ 3 H_2 O的肿瘤定位能力进行比较。并对HPLC分离组分的体外光敏化能力进行了测试。最后，通过HPLC分析给予HPD的小鼠肿瘤的卟啉提取物。随着极性的降低，各组分的肿瘤定位能力增强。虽然粗HPD在肿瘤组织中的定位程度仅与3 H2O相同，但组分7在肿瘤中的定位几乎与67 Ga一样有效。细胞对HPD组分的摄取随着极性的降低而增加。因此，低极性组分是最有效的光敏剂。
Both 3H-labeled and unlabeled hematoporphyrin derivative (HPD) were analyzed by high-pressure liquid chromatography (HPLC) and gel permeation chromatography. Four main components were isolated by HPLC, and two were isolated by gel permeation chromatography. The tumor-localizing ability of each component was tested and compared to that of 67Ga and 3H2O by injection in mice bearing Lewis lung carcinoma. The photosensitizing abilities of the HPLC-separated components in vitro were also tested. Finally, porphyrin extracts of tumors from mice given HPD were analyzed by HPLC. The tumor-localizing ability of the components increased with decreasing polarity. While crude HPD localized in tumor tissue only to the same extent as did 3H2O, Component 7 was almost as effective as was 67Ga in localizing in the tumor. The cellular uptake of HPD components increased with decreasing polarity. In accordance with this, the low-polarity components were the most effective photosensitizers.
用血卟啉衍生物检测和定位膀胱原位癌。
DOI：
--
发表时间：
1982
期刊：
Mayo Clinic proceedings. Mayo Clinic
影响因子：
--
作者：
BensonJr,RC;Farrow,GM;Kinsey,JH;Cortese,DA;Zincke,H;Utz,DC
通讯作者：
Utz,DC
鼠白血病 L1210 细胞对血卟啉衍生物和相关卟啉的转运和结合。
影响因子：
11.2
作者：
Kessel,D
通讯作者：
Kessel,D
影响因子：
11.2
作者：
Kessel,D
通讯作者：
Kessel,D