UNIQUE TISSUE DISTRIBUTION OF 2 DISTINCT CELLULAR RETINOIC ACID BINDING-PROTEINS IN NEONATAL AND ADULT-RAT
UNIQUE TISSUE DISTRIBUTION OF 2 DISTINCT CELLULAR RETINOIC ACID BINDING-PROTEINS IN NEONATAL AND ADULT-RAT
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DOI：
10.1016/0304-4165(90)90131-f
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发表时间：
1990-03-26
期刊：
影响因子：
--
通讯作者：
SIU, CH
中科院分区：
文献类型：
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作者：
BAILEY, JS;SIU, CH
在新生大鼠幼仔中发现了两种不同种类的细胞视黄酸结合蛋白，CRABP-I和CRABP-II（Bailey，J.S.和Siu，C.- H.（1988）J.Biol.Chem.263，9326-9332）。在这份报告中，我们描述了一个敏感的放射性配体结合测定粗组织提取物中的CRABP。该测定利用阴离子交换高压液相色谱法，有效地分离CRABP-II从CRABP-I，从而允许同时定量这两种蛋白质。研究了CRABP-I和CRABP-II在新生大鼠和成年大鼠各种组织中的分布。CRABP-1是CRABP的主要种类，并且在脑、皮肤和睾丸中以高水平存在。CRABP-II显然是新生动物皮肤所特有的，在成年动物皮肤中检测不到。有趣的是，CRABP-II仅在成年动物的肾上腺中以显著水平检测到，而新生儿肾上腺仅表达CRABP-I而不表达CRABP-II。CRABP-I在新生儿阶段的大多数器官中的水平高于成人。
Two different species of cellular retinoic acid binding proetins, CRABP-I and CRABP-II, have been found in neonatal rat pups (Bailey, J. S. and Siu, C.-H. (1988) J. Biol. Chem. 263, 9326-9332). In this report, we describe a sensitive radio-ligand binding assay for CRABP in crude tissue extracts. The assay makes use of anion-exchange high-pressure liquid chromatography which effectively separates CRABP-II from CRABP-I, thus permitting the simultaneous quantitation of these two proteins. The distribution CRABP-I and CRABP-II in various neonatal and adult tissues of the rat has been examined. CRABP-1 is the predominant species of CRABP and is present in high levels in the brain, skin and testis. CRABP-II is apparently unique to the skin of neonatal animals and it becomes undetectable in adult skin. Interestingly, CRABP-II is detected at a significant level only in the adrenals of adult animals, while neonatal adrenals express only CRABP-I and not CRABP-II. CRABP-I is present in higher levels in most organs at the neonatal stage than in the adult.