Nasal allergen provocation induces adhesion molecule expression and tissue eosinophilia in upper and lower airways
Nasal allergen provocation induces adhesion molecule expression and tissue eosinophilia in upper and lower airways
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DOI：
10.1067/mai.2001.113046
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发表时间：
2001-03-01
影响因子：
14.2
通讯作者：
Fokkens, WJ
中科院分区：
文献类型：
--
作者：
Braunstahl, GJ;Overbeek, SE;Fokkens, WJ
背景资料：变应性鼻炎（AR）和哮喘的特征在于通过相似的炎症过程，其中嗜酸性粒细胞是重要的效应细胞。目的：研究鼻变应原激发（nasal allergenic激发，NP）后鼻和支气管黏膜粘附分子的表达，分析鼻支气管串扰的发生情况。方法：9例非哮喘季节性变应性鼻炎患者和9例健康对照者进行非季节性NP。在NP前（T-0）和NP后24小时（T-24）采集支气管和鼻活检标本。分析粘液切片中嗜酸性粒细胞、IL-5、嗜酸性粒细胞趋化因子、细胞间粘附分子1（ICAM-1）、血管细胞粘附分子1（VCAM-1）、E-选择素和人内皮细胞（CD 31）的存在。在T-24，嗜酸性粒细胞流入鼻上皮细胞检测（P = 0.01）和固有层（P <0.01），以及支气管上皮（P = 0.05）和固有层（P <0.05）。T-24时，AR患者鼻和支气管组织中ICAM-1表达增加，ICAM-1（+）、VCAM-1（+）和E-选择素（+）血管百分比增加。粘膜嗜酸性粒细胞的数量与局部表达的ICAM-1，E-选择素，和VCAM-1在AR患者。结论：这项研究表明，NP在AR患者的结果在全身气道炎症通过上调粘附分子。
Background: Allergic rhinitis (AR) and asthma are characterized by means of a similar inflammatory process in which eosinophils are important effector cells. The migration of eosinophils from the blood into the tissues is dependent on adhesion molecules.Objective: To analyze the aspects of nasobronchial cross-talk, we studied the expression of adhesion molecules in nasal and bronchial mucosa after nasal allergen provocation (NP).Methods: Nine nonasthmatic subjects with seasonal AR and 9 healthy control subjects underwent NP out of season. Bronchial and nasal biopsy specimens were taken before (T-0) and 24 hours after NP (T-24). Mucosal sections were analyzed for the presence of eosinophils, IL-5, eotaxin, intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), E-selectin, and human endothelium (CD31).Results: At T-24, an influx of eosinophils was detected in nasal epithelium (P = .01) and lamina propria (P < .01), as well as in bronchial epithelium (P = .05) and lamina propria (P < .05), of the patients with AR. At T-24, increased expression of ICAM-1, as well as increased percentages of ICAM-1(+), VCAM-1(+), and E-selectin(+) vessels, were seen in nasal and bronchial tissue of patients with AR. The number of mucosal eosinophils correlated with the local expression of ICAM-1, E-selectin, and VCAM-1 in patients with AR.Conclusion: This study shows that NP in patients with AR results in generalized airway inflammation through upregulation of adhesion molecules.