The transcription factor MdMYB2 influences cold tolerance and anthocyanin accumulation by activating SUMO E3 ligase MdSIZ1 in apple
The transcription factor MdMYB2 influences cold tolerance and anthocyanin accumulation by activating SUMO E3 ligase MdSIZ1 in apple
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DOI：
10.1093/plphys/kiac211
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发表时间：
2022-05-17
期刊：
影响因子：
7.4
通讯作者：
Li, Yuan-Yuan
中科院分区：
文献类型：
--
作者：
Jiang, Han;Zhou, Li-Jie;Li, Yuan-Yuan
小泛素样修饰物（SUMO）肽与靶蛋白的偶联是一种重要的翻译后修饰。含有SAP和MIZ1结构域的LIGASE1 （MdSIZ1）是苹果（Malus domestica Borkh）。在逆境条件下植物生长发育过程中介导其靶点的SUMO E3连接酶。然而，目前尚不清楚MdSIZ1如何感知各种环境信号，以及sumoylation是否在转录水平上受到调节。在这项研究中，我们分析了MdSIZ1启动子，发现它含有MYB结合位点（MBS）基序，该基序对于MdSIZ1对低温和干旱的响应至关重要。随后，我们使用酵母单杂交筛选来证明MYB转录因子MdMYB2直接结合到MdSIZ1启动子中的MBS基序上。MdMYB2和MdSIZ1的表型分析表明，MdMYB2和MdSIZ1的表达可显著提高植物的耐寒性。通过LT诱导MdMYB2进一步激活MdSIZ1的表达，从而促进苹果花青素合成关键调控因子MdMYB1的sumo化。MdMYB2以依赖mdsiz1的方式促进苹果果实、苹果愈伤组织和拟南芥花青素的积累。此外，MdMYB2和MdSIZ1启动子的相互作用显著提高了植物对冷胁迫的耐受性。综上所述，我们的研究结果揭示了sumoylation转录调控的重要作用，并为植物花青素生物合成调控机制和胁迫反应提供了新的见解。一种调节苹果抗寒性和花青素积累的机制是通过MdMYB2与SUMO E3连接酶MdSIZ1的结合。
Conjugation of the small ubiquitin-like modifier (SUMO) peptide to target proteins is an important post-translational modification. SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1 (MdSIZ1) is an apple (Malus domestica Borkh). SUMO E3 ligase that mediates sumoylation of its targets during plant growth and development under adverse environmental conditions. However, it is unclear how MdSIZ1 senses the various environmental signals and whether sumoylation is regulated at the transcriptional level. In this study, we analyzed the MdSIZ1 promoter and found that it contained an MYB binding site (MBS) motif that was essential for the response of MdSIZ1 to low temperature (LT) and drought. Subsequently, we used yeast one-hybridization screening to demonstrate that a MYB transcription factor, MdMYB2, directly bound to the MBS motif in the MdSIZ1 promoter. Phenotypic characterization of MdMYB2 and MdSIZ1 suggested that the expression of both MdMYB2 and MdSIZ1 substantially improved cold tolerance in plants. MdMYB2 was induced by LT and further activated the expression of MdSIZ1, thereby promoting the sumoylation of MdMYB1, a key regulator of anthocyanin biosynthesis in apple. MdMYB2 promoted anthocyanin accumulation in apple fruits, apple calli, and Arabidopsis (Arabidopsis thaliana) in an MdSIZ1-dependent manner. In addition, the interaction of MdMYB2 and the MdSIZ1 promoter substantially improved plant tolerance to cold stress. Taken together, our findings reveal an important role for transcriptional regulation of sumoylation and provide insights into plant anthocyanin biosynthesis regulation mechanisms and stress response.A mechanism regulates cold tolerance and anthocyanin accumulation in apple through the binding of MdMYB2 to the SUMO E3 ligase MdSIZ1.