Chain conformation and anti-tumor activity of derivatives of polysaccharide from Rhizoma Panacis Japonici
Chain conformation and anti-tumor activity of derivatives of polysaccharide from Rhizoma Panacis Japonici
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DOI：
10.1016/j.carbpol.2014.01.089
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发表时间：
2014-05-25
影响因子：
11.2
通讯作者：
Wang, Kaiping
中科院分区：
文献类型：
--
作者：
Chen, Cong;Wu, Wenhua;Wang, Kaiping
从海参中提取的超支化(1-GT；4)-α-D-葡聚糖(RPS3)支化度为35%，呈球状构象，几乎没有生物活性。然后合成了三种衍生物：硫酸化(S-)、磷酸化(P-)和羧甲基化(CM-)RPS3，并用FTIR、(CNMR)-C-13和Sec-LLS-Vis-RI进行了表征。结果表明，CM-RPS3、S-RPS3和P-RPS3的相对分子质量较原RPS3急剧下降，表明发生了化学降解。此外，随着α(ETa)、α(S)和Rho的增大，RPS3的球状构象转变为无规卷曲构象。这归因于枝条优先退化的发生。体外和体内实验表明，适当低分子质量的带负电荷的S-RPS3和P-RPS3对H-22肿瘤细胞生长有明显的抑制作用。这项工作为拓宽α-D-葡聚糖的生物应用提供了有价值的结果。(C)2014爱思唯尔有限公司。保留所有权利。
A hyper branched (1 -> 4)-alpha-D-glucan (RPS3) with degree of branching of 35% extracted from Rhizoma Panacis Japonici adopted a sphere-like conformation and showed little bioactivity. Three derivatives including sulfated (S-), phosphated (P-) and carboxymethylated (CM-) RPS3 were then synthesized and characterized by FTIR, (CNMR)-C-13, and SEC-LLS-Vis-RI. As a result, the molecular weights of CM-RPS3, S-RPS3 and P-RPS3 decreased sharply in contrast to the original one, suggesting that chemical degradation has occurred. Moreover, the sphere-like conformation of RPS3 transferred into the random coil-like conformation according to the increased values of alpha(eta), alpha(s) and rho. It was ascribed to the occurrence of the preferential degradation for the branches. In vitro and in vivo tests demonstrated that the negatively charged S-RPS3 and P-RPS3 with properly low molecular mass significantly inhibited H-22 tumor cells growth. This work offered valuable results for broadening the biological applications of alpha-D-glucans. (C) 2014 Elsevier Ltd. All rights reserved.