Analysis of the bmp gene family in Borrelia burgdorferi sensu lato.
Analysis of the bmp gene family in Borrelia burgdorferi sensu lato.
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伯氏疏螺旋体 bmp 基因家族的分析。
DOI：
10.1128/jb.182.7.2037-2042.2000
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发表时间：
2000
影响因子：
3.2
通讯作者：
Cabello,FC
中科院分区：
文献类型：
--
作者：
Gorbacheva,VY;Godfrey,HP;Cabello,FC
BmpA, BmpB, BmpC, and BmpD are homologousBorrelia burgdorferilipoproteins of unknown functions, encoded by thebmpgenes of paralogous chromosomal gene family 36. At least some of the Bmp proteins are immunogens in infected vertebrate hosts. The genetic organization of thebmpregion has been characterized for a variety ofB. burgdorferisensu lato strains by Southern hybridization, PCR amplification, and DNA sequencing. All fourbmpgenes were present in the same relative order in allB. burgdorferisensu lato low- and high-passage-number isolates. While there were no differences in the relative orders of thebmpgenes in these species, variations in DNA sequence in thebmpD-bmpCandbmpC-bmpAintergenic regions were significantly more common than in the corresponding 3′bmpDandbmpCcoding regions. The genetic structure of the chromosomal region containing thebmpgenes thus appears to be well conserved across different species ofB. burgdorferi, but variations in DNA fine structure that prevent PCR primer annealing may occur in this region and make Southern hybridization much more reliable than PCR for detection of the presence of these genes. Our results also suggest thatbmpgene products may be used as reagents in the preparation of vaccines and diagnostic assays to protect against and diagnose Lyme disease produced byB. burgdorferisensu lato.
