Characterization of infiltrating T cells and Th1/Th2-type cytokines in the synovium of patients with osteoarthritis
Characterization of infiltrating T cells and Th1/Th2-type cytokines in the synovium of patients with osteoarthritis
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DOI：
10.1053/joca.2001.0509
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发表时间：
2002-04-01
影响因子：
7
通讯作者：
Yoshino, S
中科院分区：
文献类型：
--
作者：
Ishii, H;Tanaka, H;Yoshino, S
目的：骨关节炎（OA）是由机械应力引起的软骨破坏所致，与类风湿性关节炎（RA）相比，OA与免疫反应的关系不大。我们先前已经发现OA患者滑膜中T细胞受体（TCR）的V β链的克隆扩增转录本。为了检验OA中涉及免疫应答的假设，我们确定：（a）OA患者的滑膜中是否有CD 3（+）、CD 4（+）和CD 8（+）T细胞浸润;（B）OA患者滑膜中蛋白水平产生的Th 1/Th 2型细胞因子。免疫组织化学分析是通过使用针对CD 3（+）、CD 4（+）和CD 8（+）T细胞差异抗原的特异性抗体来鉴定OA患者滑膜中浸润的T细胞，结果：OA患者滑膜下层CD 4（+）T细胞较正常人滑膜下层明显增多。OA患者滑膜组织中IFN-γ（+）细胞数量明显高于IL-4（+）细胞（P
Objective: It has been suggested that osteoarthritis (OA) is induced by mechanical stress followed by cartilage destruction, and it is generally accepted that there is little involvement of an immune response in OA compared with that in rheumatoid arthritis (RA). We have previously found clonally expanded transcripts of Vbeta chain of the T cell receptor (TCR) in the synovium of patients with OA. To test the hypothesis that an immune response is involved in OA, we determined: (a) whether CD3(+), CD4(+), and CD8(+) T-cells were infiltrating the synovial membrane of patients with OA; (b) the Th1/Th2-type cytokines produced at the protein level in the synovium of patients with OA.Methods: Immunohistochemical analysis was performed to identify T-cells that infiltrated the synovium of patients with OA using specific antibodies against CD3(+), CD4(+), and CD8(+) T-cell differential antigens, interferon-gamma (IFN-gamma as a marker for Th1 cells, and interleukin-4 (IL-4) as a marker for Th2 cells.Results: CD4(+) T-cells were strongly detected in the sublining layer of the synovium of patients with OA compared with the number detected in the same synovial layer of normal subjects. The number of IFN-gamma(+) cells was significantly higher than that of IL-4(+) cells in the synovium of patients with OA (P