The effect of cells transferred into the mouse blastocyst on subsequent development.
The effect of cells transferred into the mouse blastocyst on subsequent development.
复制标题
细胞的影响转移到小鼠胚泡中对随后的发育。
DOI：
10.1084/jem.140.4.1049
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发表时间：
1974-10-01
影响因子：
15.3
通讯作者：
Brinster, R L
中科院分区：
文献类型：
--
作者：
Brinster, R L
将来自CBA T6 T6小鼠的骨髓细胞和来自129只SvSl小鼠的睾丸畸胎癌细胞转移到来自随机繁殖的Swiss白化病小鼠的胚泡中。使胚泡在寄养母体中发育，并研究由这些胚泡产生的成体，以证明转移细胞的作用。总共有137只成年小鼠从实验中产生，其中一只在胚泡阶段接受畸胎瘤细胞的小鼠显示出几条细细的绒毛。所有的成年动物都接受了与提供细胞的动物相同的皮肤移植。在所有的情况下，由胚泡产生的动物，其中细胞已被转移到维持皮肤移植显着更长的时间比对照组。在一些病例中，移植物产生了粘毛，在两个病例中，移植物维持了大约2个月。这些实验表明，转移的细胞能够在胚胎中建立小的集落，其中一些细胞持续到成年。
Bone marrow cells from CBA T6T6 mice and testicular teratocarcinoma cells from 129 SvSl mice were transferred into blastocysts from random-bred Swiss albino mice. The blastocysts were allowed to develop in foster mothers and the adults resulting from these blastocysts were studied for evidence of an effect of the transferred cells. A total of 137 adults resulted from the experiments, and one of the mice that had received teratocarcinoma cells in the blastocyst stage showed several thin stripes of agouti hair. All the adult animals received grafts of skin from animals identical to those supplying the cells. In all cases the animals that resulted from blastocysts into which cells had been transferred maintained skin grafts for a significantly longer period than controls. In a number of cases the graft developed agouti hair and in two cases the graft was maintained for approximately 2 mo. These experiments indicate that the transferred cells were able to establish small colonies in the embryos and that some of these cells persisted into the adult.