An accessible micro-capillary electrophoresis device using surface-tension-driven flow.
An accessible micro-capillary electrophoresis device using surface-tension-driven flow.
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一种使用表面张力驱动流动的易于使用的微毛细管电泳装置。
DOI：
10.1002/elps.200800595
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发表时间：
2009
期刊：
影响因子：
2.9
通讯作者：
Beebe,DavidJ
中科院分区：
文献类型：
--
作者：
Mohanty,SwomitraK;Warrick,Jay;Gorski,Jack;Beebe,DavidJ
对于0.5 mm宽的微毛细管电泳芯片，将0.33 μL 5μM溶液（浓度先前由威斯康星大学生物技术中心寡核苷酸合成中心测定）输送到分离通道中。使用维度分析并给出该特定寡核苷酸的MW为31185μg/mol：(0.33 x 10-6 L) x (5 x 10-6 mol/1 x 10-6 L) x (31185μg/mol)= 0.514 μg或51.4 ng计算显示51.4 ng DNA被注入到装置中。使用被动泵从设备中提取样品，并将其放入 100μL 液滴中，并在 Biotek HT 荧光板读数器中进行分析。获得的读数为 45。使用上面的拟合方程，100μl 滴中提取的寡核苷酸的浓度为 0.0137 μM。再次使用量纲分析：
For the 0.5 mm wide micro capillary electrophoresis chip, 0.33 μL of a 5μM solution (concentration was previously determined by oligonucleotide synthesis center, University of Wisconsin Biotechnology Center) was delivered into the separation channel. Using dimensional analysis and given the MW of this particular oligonucleotide was 31185μg/mol:(0.33 x 10-6 L) x (5 x 10-6 mol/1 x 10-6 L) x (31185μg/mol)= 0.514 μg or 51.4 ngThe calculation shows 51.4 ng of DNA was injected into the device. The sample was extracted from the device using passive pumping and was placed in a 100μL drop and analyzed in a Biotek HT fluorescent plate reader. The reading obtained was 45. Using the fit equation above, the concentration of extracted oligonucleotide in the 100μl drop was 0.0137 μM. Once again using dimensional analysis: