High loads of nano-hydroxyapatite/graphene nanoribbon composites guided bone regeneration using an osteoporotic animal model
High loads of nano-hydroxyapatite/graphene nanoribbon composites guided bone regeneration using an osteoporotic animal model
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DOI：
10.2147/ijn.s192456
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发表时间：
2019-01-01
影响因子：
8
通讯作者：
Lobo, Anderson Oliveira
中科院分区：
文献类型：
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作者：
Oliveira, Francilio Carvalho;Carvalho, Jancineide Oliveira;Lobo, Anderson Oliveira
背景：对于骨质疏松症患者，很难找到能够优化骨修复治疗和充分骨整合的生物活性化合物。具有氧化石墨烯的纳米羟基磷灰石(nHAp)/碳纳米管，被称为石墨烯纳米带（GNR）复合材料，由于其生物活性和骨整合特性而成为骨再生的有前途的材料/支架。在此，我们通过骨质疏松模型评估了nHAp/GNR复合材料（nHAp/GNR）促进骨再生的作用。材料和方法：首先，制备了三种不同的nHAp/GNR （GNR的1、2和3 wt%）并对其进行了表征。活体实验采用36只Wistar大鼠（var. albinus，体重250-300 g， Comissao de Etica no Uso de Animais [CEUA] n.002/17）。植入前，雌性大鼠卵巢切除术诱导骨质疏松。45天后，使用3.0 mm Quest环钻造成胫骨骨折。然后，在21天和45天的两个不同时间段将这些动物分成6个样本组。病变部位用刮管填充3mg上述样品之一。在植入21或45天后，这些动物被安乐死以进行分析。进行组织学、生化和放射学分析（DIGORA法）。通过方差分析、Tukey检验和Kolmogorov-Smirnov检验对数据进行评估，在P2%>1% wt下具有统计学意义。结论：因此可以推断，所有分析的纳米颗粒都促进了骨质疏松大鼠的骨再生，与分析时间无关。
Background: It has been difficult to find bioactive compounds that can optimize bone repair therapy and adequate osseointegration for people with osteoporosis. The nano-hydroxyapatite (nHAp)/carbon nanotubes with graphene oxides, termed graphene nanoribbons (GNR) composites have emerged as promising materials/scaffolds for bone regeneration due to their bioactivity and osseointegration properties. Herein, we evaluated the action of nHAp/GNR composites (nHAp/GNR) to promote bone regeneration using an osteoporotic model.Materials and methods: First, three different nHAp/GNR (1, 2, and 3 wt% of GNR) were produced and characterized. For in vivo analyses, 36 Wistar rats (var. albinus, weighing 250-300 g, Comissao de Etica no Uso de Animais [CEUA] n.002/17) were used. Prior to implantation, osteoporosis was induced by oophorectomy in female rats. After 45 days, a tibial fracture was inflicted using a 3.0-mm Quest trephine drill. Then, the animals were separated into six sample groups at two different time periods of 21 and 45 days. The lesions were filled with 3 mg of one of the above samples using a curette. After 21 or 45 days of implantation, the animals were euthanized for analysis. Histological, biochemical, and radiographic analyses (DIGORA method) were performed. The data were evaluated through ANOVA, Tukey test, and Kolmogorov-Smirnov test with statistical significance at P2%>1% wt.Conclusion: Therefore, it can be inferred that all analyzed nanoparticles promoted bone regeneration in osteoporotic rats independent of analyzed time.