Organization of subcortical auditory nuclei of Japanese house bat (Pipistrellus abramus) identified with cytoarchitecture and molecular expression
Organization of subcortical auditory nuclei of Japanese house bat (Pipistrellus abramus) identified with cytoarchitecture and molecular expression
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DOI：
10.1002/cne.24529
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发表时间：
2018-12-01
影响因子：
2.5
通讯作者：
Riquimaroux, Hiroshi
中科院分区：
文献类型：
--
作者：
Ito, Tetsufumi;Furuyama, Takafumi;Riquimaroux, Hiroshi
回声定位蝙蝠的听觉系统表现出显着的专门化，可能与分析声纳脉冲的回声有关。然而，回声定位蝙蝠之间在听觉通路的组织上存在显著的种间差异，并且听觉核团与非回声定位蝙蝠的同源性尚未建立。为了建立回声定位蝙蝠听觉通路的同源性和专一性，本研究对家蝠皮质下听觉核团中谷氨酸、氨基丁酸和甘氨酸能三种表型标志物的表达进行了研究。在上橄榄复合体中，我们发现内侧上橄榄核和上橄榄旁核分别表达谷氨酸和GABA能表型，这表明这些核团与啮齿动物的同源。在外侧丘核内，背核为纯GABA能，中间核为谷氨酸和抑制性神经元的混合体，腹侧核的致密部分为单纯甘氨酸能，腹侧核的多极部分同时表达GABA和甘氨酸。在下丘，根据终末密度和大型GABA能神经元形态的不同，中央核进一步分为背部和腹侧部，提示声纳脉冲结构的专门化。内侧膝状体实质上缺乏GABA能神经元，提示顶盖丘脑通路的组织结构与啮齿动物相似。综上所述，我们的发现表明，特化主要发生在核的大小和NLL和IC的组织方面。
The auditory system of echolocating bats shows remarkable specialization likely related to analyzing echoes of sonar pulses. However, significant interspecies differences have been observed in the organization of auditory pathways among echolocating bats, and the homology of auditory nuclei with those of non-echolocating species has not been established. Here, in order to establish the homology and specialization of auditory pathways in echolocating bats, the expression of markers for glutamatergic, GABAergic, and glycinergic phenotypes in the subcortical auditory nuclei of Japanese house bat (Pipistrellus abramus) was evaluated. In the superior olivary complex, we identified the medial superior olive and superior paraolivary nuclei as expressing glutamatergic and GABAergic phenotypes, respectively, suggesting these nuclei are homologous with those of rodents. In the nuclei of the lateral lemniscus (NLL), the dorsal nucleus was found to be purely GABAergic, the intermediate nucleus was a mixture of glutamatergic and inhibitory neurons, the compact part of the ventral nucleus was purely glycinergic, and the multipolar part of the ventral nucleus expressed both GABA and glycine. In the inferior colliculus (IC), the central nucleus was found to be further subdivided into dorsal and ventral parts according to differences in the density of terminals and the morphology of large GABAergic neurons, suggesting specialization to sonar pulse structure. Medial geniculate virtually lacked GABAergic neurons, suggesting that the organization of the tectothalamic pathway is similar with that of rodents. Taken together, our findings revealed that specialization primarily occurs with regard to nuclei size and organization of the NLL and IC.