Inhibin-like activity in Sertoli cell culture media and testicular homogenates from rats of various ages.
Inhibin-like activity in Sertoli cell culture media and testicular homogenates from rats of various ages.
复制标题
支持细胞培养基和不同年龄大鼠睾丸匀浆中的抑制素样活性。
DOI：
10.1677/joe.0.1130103
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发表时间：
1987
影响因子：
4
通讯作者：
F. Jong
中科院分区：
文献类型：
--
作者：
A. U. Gessel;F. Jong
通过体内和体外实验，研究了年龄对未治疗、新生儿期半月龄和产前照射大鼠睾丸抑制素的影响。在1日龄、7日龄、14日龄、21日龄、42日龄和63日龄大鼠制备的睾丸胞浆中，可用分散的垂体腺细胞体外生物测定法测定睾丸抑制素的浓度。睾丸胞浆制剂对垂体FSH的分泌呈剂量依赖性抑制，而对促黄体生成素的分泌无明显影响。随着年龄的增长，睾丸抑制素含量逐渐增加，而在14日龄后，各试验组的外周FSH含量均有较大幅度的增加。新生儿半去势或产前照射可导致睾丸抑制素含量降低，血浆FSH水平升高。于培养第3天测定7、14、21、42和63日龄正常大鼠的支持细胞在培养24小时内产生抑制素的活性。每10(6)个支持细胞的抑制素产量在14日龄后迅速下降，63日龄大鼠支持细胞的抑制素产量最低。在与绵羊FSH预先孵育后，21日龄大鼠睾丸的废液中检测到明显更多的抑制素活性。相反，在所研究的大多数年龄段，在睾丸激素存在的预培养后，抑制素的产生被发现。这些来自体内和体外实验的数据表明，在21日龄之前，脑垂体FSH的分泌和抑制素的产生之间存在着相互作用的关系。
The influence of age on testicular inhibin in untreated, neonatally hemicastrated and prenatally irradiated rats was studied using in-vivo and in-vitro experiments. In testicular cytosols prepared from 1-, 7-, 14-, 21-, 42- and 63-day-old rats concentrations of testicular inhibin could be measured with an in-vitro bioassay method using dispersed pituitary cells. Preparations of testicular cytosols caused a dose-dependent suppression of pituitary FSH secretion, whereas no effects were found on LH secretion. Testicular content of inhibin increased gradually with age, while after 14 days of age a relatively large increase of peripheral FSH concentrations occurred in all experimental groups. Neonatal hemicastration or prenatal irradiation resulted in decreased inhibin content of the testis and increased plasma FSH levels. The production of inhibin activity by Sertoli cells obtained from 7-, 14-, 21-, 42- and 63-day-old normal rats was measured during a 24-h incubation period on the third day of culture. The inhibin production per 10(6) plated Sertoli cells decreased rapidly after 14 days of age and the lowest production of inhibin was found in Sertoli cells from rats of 63 days of age. After pre-incubation with ovine FSH significantly larger amounts of inhibin activity were detected in spent media from 21-day-old rat testes. In contrast, suppression of inhibin production was found after pre-culture in the presence of testosterone at most of the ages studied. These data from in-vivo and in-vitro experiments indicate that a reciprocal relationship exists between pituitary FSH secretion and inhibin production before the age of 21 days.(ABSTRACT TRUNCATED AT 250 WORDS)