Effects of dihydroquercetin obtained from deodar (Cedrus deodara) on immune status of gilthead seabream (Sparus aurata L.)
Effects of dihydroquercetin obtained from deodar (Cedrus deodara) on immune status of gilthead seabream (Sparus aurata L.)
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DOI：
10.1016/j.fsi.2014.12.009
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发表时间：
2015-03-01
影响因子：
4.7
通讯作者：
Esteban, M. Angeles
中科院分区：
文献类型：
--
作者：
Awad, Elham;Awaad, Amani S.;Esteban, M. Angeles
在鱼类中使用药用植物作为预防方法被认为是安全的，是水产养殖实践中使用化学品的一种非常有前途的替代方法。研究了药用植物雪松（Cedrus deodara）提取物二氢槲皮素（dihydroquercetin）对金头鲷（Sparus aurata L.）免疫功能的影响。将鱼分为4组，分别饲喂0%（对照）、0.1%、0.5%和1%二氢槲皮素的商品饲料14天。细胞（吞噬和呼吸爆发活动）和体液（血清补体活性，抗蛋白酶，总蛋白，过氧化物酶，杀菌活性和IgM水平）的免疫参数进行了研究。与对照组相比，结果记录了所有测试参数和所有二氢槲皮素补充组的增强。有趣的是，与对照相比，接受最低剂量的二氢槲皮素（0.1%）的鱼在吞噬作用、呼吸爆发、IgM水平、总蛋白、补体、抗蛋白酶和杀菌活性方面显示出高度显著的差异（p < 0.05）。在先前的体外试验中，还研究了不同剂量的二氢槲皮素对头肾白细胞的直接作用。同样，检测的最低剂量激发了最高的免疫细胞活性，其中，在分别用0.025%和0.0125%剂量孵育的白细胞中记录了最高的吞噬和呼吸爆发活性。因此，研究结果表明，低浓度的二氢槲皮素作为食物补充剂能够提高金头鲷的免疫状态。(C)2014爱思唯尔有限公司版权所有。
The use of medicinal plants as prophylactic method in fish is considered safe and a very promising alternative to the use of chemicals in aquaculture practices. The prospective mode of action of dihydroquercetin, fraction of the medical plant deodar (Cedrus deodara), was evaluated on immune status of gilthead seabream (Sparus aurata L). Fish were divided into 4 groups before being fed for 14 days with commercial diets supplemented with 0% (control), 0.1%, 0.5% and 1% of dihydroquercetin. Cellular (phagocytosis and respiratory burst activities) and humoral (seric complement activity, antiprotease, total protein, peroxidase, bactericidal activity and IgM level) immune parameters were investigated. The results recorded enhancement in all the tested parameters and in all the dihydroquercetin supplemented groups compared to the control. Interestingly, the fish received the lowest dose of dihydroquercetin (0.1%) showed a highly significant difference (p < 0.05) in phagocytosis, respiratory burst, IgM level, total protein, complement, antiprotease and bactericidal activities compared to the control. Direct effect of different doses of dihydroquercetin on head-kidney leucocytes was also studied in a previous in vitro assay. Again, the lowest doses tested provoked the highest immune cellular activities, where, the highest phagocytic and respiratory bust activities were recorded in leucocytes incubated with 0.025% and 0.0125% doses, respectively. Therefore, the results suggest that low concentrations of dihydroquercetin as food supplements are able to increase the immune status of gilthead seabream. (C) 2014 Elsevier Ltd. All rights reserved.