A guinea-pig model of Hendra virus encephalitis.
A guinea-pig model of Hendra virus encephalitis.
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亨德拉病毒脑炎豚鼠模型。
DOI：
10.1053/jcpa.2001.0464
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发表时间：
2001
影响因子：
0.8
通讯作者：
R. Slocombe
中科院分区：
文献类型：
--
作者：
M. Williamson;P. Hooper;P. Selleck;H. Westbury;R. Slocombe
皮下接种，而不是皮内（足垫）或鼻内接种，与高剂量的亨德拉病毒（HeV）的一致性产生疾病的豚鼠。在15只皮下接种的动物中，14只发生血管疾病，在一系列组织中有阳性HeV免疫组化标记。一个新的观察结果是存在病变，包括合胞体细胞，在膀胱移行上皮免疫标记。从尿液而非鼻、口腔、直肠或结膜拭子（其他外部部位）中分离出病毒，与先前在马中进行的流行病学研究一致，表明传播的可能性有限。所用剂量（30 000至50 000 TCID（50））高于先前的研究，在15只皮下接种的豚鼠中，有8只出现脑炎的显微镜病变，血管和神经元免疫标记阳性，尤其是在髓质、小脑和丘脑。病毒是从六个患脑炎的大脑中回收的。严重的血管变性的中心，脑炎病变的8只豚鼠和阳性免疫标记的脉络丛的进一步的动物表明，病毒进入大脑后，病毒引起的血管损伤和脉络丛的入侵。豚鼠似乎适合于研究HeV脑炎。
Subcutaneous inoculation, but not intradermal (footpad) or intranasal inoculation, with high doses of Hendra virus (HeV) consistently produced disease in guinea-pigs. Of 15 subcutaneously inoculated animals, 14 developed vascular disease with positive HeV immunohistochemical labelling in a range of tissues. A new observation was the presence of lesions, including syncytial cells, with immunolabelling in the transitional epithelium of the bladder. Virus isolation from the urine rather than from nasal, oral, rectal or conjunctival swabs, the other external sites, was consistent with previous epidemiological work in horses, indicating a limited possibility of transmission. The dose used (30 000 to 50 000 TCID(50)), which was higher than in previous studies, produced microscopical lesions of encephalitis in eight of the 15 subcutaneously inoculated guinea-pigs, with positive immunolabelling in blood vessels and neurons, especially in the medulla, cerebellum and thalamus. The virus was recovered from six of the encephalitic brains. Severe vascular degeneration in the centres of encephalitic lesions in six of the eight encephalitic guinea-pigs and positive immunolabelling in the choroid plexus of a further animal indicated that the virus entered the brain following virus-induced vascular injury and choroid plexus invasion. Guinea-pigs would appear to be suitable for the study of HeV encephalitis.