Determination of human cytomegalovirus genetic diversity in different patient populations in Costa Rica
Determination of human cytomegalovirus genetic diversity in different patient populations in Costa Rica
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DOI：
10.1590/s0036-46652004000200006
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发表时间：
2004-04-01
期刊：
影响因子：
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通讯作者：
Herrero-Uribe, Libia
中科院分区：
文献类型：
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作者：
Ahumada-Ruiz, Sara;Taylor-Castillo, Lizeth;Herrero-Uribe, Libia
在哥斯达黎加，成人中HCMV的血清阳性率大于95%;原发性感染发生在生命早期，是新生儿中最常见的先天性感染。本研究的目的是确定哥斯达黎加人巨细胞病毒GB基因的遗传变异和基因型。样本收集自酗酒者、孕妇、献血者、艾滋病患者、血液肿瘤学（HO）儿童和巨细胞包涵体病新生儿的HCMV分离株。采用半巢式PCR方法扩增gB基因293-296 bp的片段，进行单链构象多态性（SSCP）分析和测序，分别确定gB基因的遗传多态性和基因型。艾滋病患者的多态性最高，有14种不同的多态性，而HO儿童样本中有五十六%的多态性相同，提示该组可能存在医院感染。在新生儿中检测到三种基因型（gB 1、gB 2和gB 3），而AIDS患者和献血员仅检测到一种基因型（gB 2）。在所有分析的样本中，仅确定了基因型gB 1、2和3，基因型gB 2最常见（73%），未检测到混合感染。研究结果表明，SSCP可能是检测HCMV院内感染的重要工具，并建议需要纳入额外的研究人群，以更好地确定基因型多样性和患病率。
Seroprevalence of HCMV in Costa Rica is greater than 95% in adults; primary infections occur early in life and is the most frequent congenital infection in newborns. The objectives of this study were to determine the genetic variability and genotypes of HCMV GB gene in Costa Rica. Samples were collected from alcoholics, pregnant women, blood donors, AIDS patients, hematology-oncology (HO) children and HCMV isolates from neonates with cytomegalic inclusion disease. A semi-nested PCR system was used to obtain a product of 293-296 by of the gB gene to be analyzed by Single Stranded Conformational Polymorphism (SSCP) and sequencing to determine the genetic polymorphic pattern and genotypes, respectively. AIDS patients showed the highest polymorphic diversity with 14 different patterns while fifty-six percent of HO children samples showed the same polymorphic pattern, suggesting in this group a possible nosocomial infection. In neonates three genotypes (gB 1, gB2 and gB3), were determined while AIDS patients and blood donors only showed one (gB2). Of all samples analyzed only genotypes gB 1, 2 and 3 were determined, genotype gB2 was the most frequent (73%) and mixed infections were not detected. The results of the study indicate that SSCP could be an important tool to detect HCMV intra-hospital infections and suggests a need to include additional study populations to better determine the genotype diversity and prevalence.