The initiation of spermiogenesis in the nematode Caenorhabditis elegans.
The initiation of spermiogenesis in the nematode Caenorhabditis elegans.
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DOI：
10.1016/0012-1606(83)90336-6
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发表时间：
1983-07
影响因子：
2.7
通讯作者：
S. Ward;E. Hogan;G. A. Nelson
中科院分区：
文献类型：
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作者：
S. Ward;E. Hogan;G. A. Nelson
线虫的精子发生涉及到无柄球形精子细胞活化为能动的双极变形虫精子。在秀丽隐杆线虫中，精子发生通常由交配诱导。转移到雌雄同体的精细胞以及一些留在雄性体内的精细胞在交配后几分钟变成精子。离子载体莫能菌素（G. A.纳尔逊和S. Ward，1980，Cell 19，457-464）和弱碱如三乙醇胺。三乙醇胺和莫能菌素均引起细胞内pH从7.1快速增加至7.5或8.0。这种pH值的增加先于随后的精子发生的形态学事件。三乙醇胺或莫能菌素必须存在于整个精子发生的所有细胞形成伪足，但一旦伪足形成的诱导剂是不必要的后续运动。pH诱导的精子生成被阻断线粒体或糖酵解的药物抑制。蛋白酶处理也可以诱导精子发生而不增加细胞内pH，显然绕过了激活中的pH依赖性步骤和糖酵解的要求。这些结果表明，C.与卵激活和海胆精子运动的启动中的一些步骤一样，可以通过细胞内pH的增加来诱导elegans，但是这种pH变化可以通过蛋白水解绕过。
Spermiogenesis in nematodes involves the activation of sessile spherical spermatids to motile bipolar amoeboid spermatozoa. InCaenorhabditis elegansmales spermiogenesis is normally induced by copulation. Spermatids transferred to hermaphrodites as well as some of those left behind in the male become spermatozoa a few minutes after mating. Spermiogenesis can also be inducedin vitroby the ionophore monensin (G. A. Nelson and S. Ward, 1980,Cell19,457–464) and by weak bases such as triethanolamine. Both triethanolamine and monensin cause a rapid increase in intracellular pH from 7.1 to 7.5 or 8.0. This pH increase precedes the subsequent morphological events of spermiogenesis. Triethanolamine or monensin must be present throughout spermiogenesis for all cells to form pseudopods, but once pseudopods are formed the inducers are unnecessary for subsequent motility. The pH induced spermiogenesis is inhibited by drugs that block mitochondria or glycolysis. Protease treatment can also induce spermiogenesis without increasing intracellular pH, apparently bypassing the pH-dependent steps in activation and the requirement for glycolysis. These results show that the initiation of spermiogenesis inC. elegans, like some steps in egg activation and the initiation of sea urchin sperm motility, can be induced by an increase in intracellular pH, but this pH change can be bypassed by proteolysis.