Automated calibration of optoPlate LEDs to reduce light dose variation in optogenetic experiments.
Automated calibration of optoPlate LEDs to reduce light dose variation in optogenetic experiments.
复制标题
自动校准 optoPlate LED 以减少光遗传学实验中的光剂量变化。
DOI：
10.2144/btn-2020-0077
复制
发表时间：
2020-10
期刊：
影响因子：
2.7
通讯作者：
McClean MN
中科院分区：
文献类型：
--
作者：
Grødem EO;Sweeney K;McClean MN
光遗传学系统使用光来精确控制和研究细胞过程。直到最近，还没有几种仪器可用于将受控的光剂量应用于细胞培养。optoPlate是一种由192个LED组成的可编程阵列，旨在满足这一需求。然而，LED性能各不相同，并且在没有校准的情况下，optoPlate上的LED之间存在显著的亮度差异。在这里，我们提出了一种用于校准optoPlate的方法，该方法使用可编程显微镜载物台和光功率计来自动测量optoPlate的所有192个LED。所得到的亮度测量结果用于计算校准值，该校准值调节提供给每个optoPlate LED的电流，以减少光遗传学实验中的亮度变化。可编程显微镜载物台和光功率计用于自动测量optoPlate的所有192个LED。所得到的亮度测量结果用于计算校准值，该校准值调节供应给每个LED的电流以减少LED之间的亮度变化。
Optogenetic systems use light to precisely control and investigate cellular processes. Until recently, there had been few instruments available for applying controlled light doses to cultures of cells. The optoPlate, a programmable array of 192 LEDs, was developed to meet this need. However, LED performance varies, and without calibration there are substantial brightness differences between LEDs on an optoPlate. Here we present a method for calibrating an optoPlate that uses a programmable microscope stage and optical power meter to automatically measure all 192 LEDs of an optoPlate. The resulting brightness measurements are used to calculate calibration values that tune the electrical current supplied to each optoPlate LED to reduce brightness variation in optogenetic experiments. A programmable microscope stage and optical power meter are used to automatically measure all 192 LEDs of an optoPlate. The resulting brightness measurements are used to calculate calibration values that tune the electrical current supplied to each LED to reduce brightness variation between LEDs.