Characterization and Quantification of Polyphenols and Triterpenoids in Thinned Young Fruits of Ten Pear Varieties by UPLC-Q TRAP-MS/MS
Characterization and Quantification of Polyphenols and Triterpenoids in Thinned Young Fruits of Ten Pear Varieties by UPLC-Q TRAP-MS/MS
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采用 UPLC-Q TRAP-MS/MS 表征和定量 10 个梨品种疏苗幼果中的多酚和三萜类化合物
DOI：
10.3390/molecules24010159
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发表时间：
2019-01-01
期刊：
影响因子：
4.6
通讯作者：
Zhang, Shaoling
中科院分区：
文献类型：
--
作者：
Sun, Liqiong;Tao, Shutian;Zhang, Shaoling
大量细小的梨是生物活性化合物的天然来源，在许多果园作为农业副产品被丢弃。因此，对10个稀疏幼梨品种的化学成分和抗氧化能力进行了系统的研究。采用超高效液相色谱-电喷雾电离三重四极杆(UPLC-Q TRAP-MS/MS)联用技术，在短时间内对102种多酚类化合物和16种三萜类化合物进行了定性和定量。随后，用DPPH法测定了这些梨的抗氧化能力，并分析了总抗氧化活性与各组分的相关性。结果表明，稀释梨的生物活性物质含量和抗氧化能力都较高。在化学成分上，绿原酸、奎宁酸和熊果苷是主要的多酚类物质，熊果酸是主要的三萜类化合物，而27种多酚类化合物，特别是绿原酸和大部分黄烷醇是主要的抗氧化剂。这些研究结果为进一步利用梨果副产品提供了科学依据。
Large quantities of thinned young pears, a natural source of bioactive compounds, are abandoned as agricultural by-products in many orchards. Hence, ten thinned young pear varieties were systematically investigated in terms of their chemical composition and antioxidant potential. Through ultra-performance liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry (UPLC-Q TRAP-MS/MS), 102 polyphenols and 16 triterpenoids were identified and individually quantified within a short time using multiple reaction monitoring (MRM). Subsequently, the antioxidant capacities of these pears were determined with DPPH assays, and the correlation between total antioxidant activity and each component was analyzed. The results indicated that the bioactive compound content and antioxidant capacity in thinned pears were considerably high. Regarding chemical composition, chlorogenic acid, quinic acid and arbutin were the primary polyphenols and ursolic acid was the predominant triterpenoid, whereas 27 polyphenolic compounds, especially chlorogenic acid and most of the flavan-3-ols, were the main antioxidants in young pears. These findings should provide a scientific basis for the further use of pear fruit by-products.