Monobodies and other synthetic binding proteins for expanding protein science.
Monobodies and other synthetic binding proteins for expanding protein science.
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DOI：
10.1002/pro.3148
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发表时间：
2017-05
期刊：
影响因子：
--
通讯作者：
Koide S
中科院分区：
文献类型：
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作者：
Sha F;Salzman G;Gupta A;Koide S
合成的结合蛋白是使用非抗体分子支架构建的。在过去的二十年中，对合成结合蛋白的深入结构和功能分析改进了组合文库设计和选择策略，从而产生了有效的平台，这些平台能够持续产生针对不同靶点的结合蛋白，其亲和力和特异性可与抗体相媲美。合成结合蛋白的有利属性，如小尺寸，不形成二硫键和易于制备融合蛋白，使其在蛋白质科学，细胞生物学等领域具有独特的应用。在这里，我们回顾了最近的研究，说明如何合成结合蛋白是强大的探针，可以直接连接结构和功能，往往导致新的机制的见解。我们认为，合成蛋白质将成为蛋白质科学、生物技术和医学等不同领域的强大标准工具。
Synthetic binding proteins are constructed using nonantibody molecular scaffolds. Over the last two decades, in‐depth structural and functional analyses of synthetic binding proteins have improved combinatorial library designs and selection strategies, which have resulted in potent platforms that consistently generate binding proteins to diverse targets with affinity and specificity that rival those of antibodies. Favorable attributes of synthetic binding proteins, such as small size, freedom from disulfide bond formation and ease of making fusion proteins, have enabled their unique applications in protein science, cell biology and beyond. Here, we review recent studies that illustrate how synthetic binding proteins are powerful probes that can directly link structure and function, often leading to new mechanistic insights. We propose that synthetic proteins will become powerful standard tools in diverse areas of protein science, biotechnology and medicine.