Genomic sequence analysis and characterization of Sneathia amnii sp. nov.
Genomic sequence analysis and characterization of Sneathia amnii sp. nov.
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DOI：
10.1186/1471-2164-13-s8-s4
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发表时间：
2012
期刊：
影响因子：
4.4
通讯作者：
Jefferson KK
中科院分区：
文献类型：
--
作者：
Harwich MD Jr;Serrano MG;Fettweis JM;Alves JM;Reimers MA;Vaginal Microbiome Consortium (additional members);Buck GA;Jefferson KK
Sneathia属的细菌正在成为女性生殖道的潜在病原体。Sneathia的物种，以前与Leptotrichia分组，可以是男性和女性泌尿生殖道正常微生物群的一部分，但它们也与各种临床病症相关，包括细菌性阴道病，先兆子痫，早产，自然流产，产后菌血症和其他侵入性感染。Sneathia物种也表现出与性传播疾病和宫颈癌的显着相关性。由于Sneathia物种是挑剔的，很少在体外成功培养;和属的成员的基因组到现在为止还没有被表征，很少有人知道这些生物体的生理或毒力。在这里，我们描述了一个新的物种，Sneathia sp. nov，它非常类似于以前指定的细菌“Leptotrichia pastonii”。作为VCU阴道人类微生物组项目的一部分，一种阴道分离的S。鉴定、培养和细菌学克隆了一种新的嗜热链球菌（Escherichia sp. nov.）对该菌的生化特性和毒力特性进行了体外检测，并对该菌的基因组进行了测序、注释和分析。分析揭示了约1.34 Mbp的简化环状基因组，包含约1，282个蛋白质编码基因。代谢重建的细菌反映了其生化表型，和几个基因可能与致病性进行了鉴定。具有复杂生长要求的细菌通常仍然缺乏特征，因此，它们在健康和疾病中的作用尚不清楚。阐明了该菌的生理特性，并鉴定了与代谢和毒力相关的致病基因。Escherichi可能导致更好地了解这种潜在病原体在细菌性阴道病，早产以及与阴道和生殖健康相关的其他问题中的作用。
Bacteria of the genus Sneathia are emerging as potential pathogens of the female reproductive tract. Species of Sneathia, which were formerly grouped with Leptotrichia, can be part of the normal microbiota of the genitourinary tracts of men and women, but they are also associated with a variety of clinical conditions including bacterial vaginosis, preeclampsia, preterm labor, spontaneous abortion, post-partum bacteremia and other invasive infections. Sneathia species also exhibit a significant correlation with sexually transmitted diseases and cervical cancer. Because Sneathia species are fastidious and rarely cultured successfully in vitro; and the genomes of members of the genus had until now not been characterized, very little is known about the physiology or the virulence of these organisms. Here, we describe a novel species, Sneathia amnii sp. nov, which closely resembles bacteria previously designated "Leptotrichia amnionii". As part of the Vaginal Human Microbiome Project at VCU, a vaginal isolate of S. amnii sp. nov. was identified, successfully cultured and bacteriologically cloned. The biochemical characteristics and virulence properties of the organism were examined in vitro, and the genome of the organism was sequenced, annotated and analyzed. The analysis revealed a reduced circular genome of ~1.34 Mbp, containing ~1,282 protein-coding genes. Metabolic reconstruction of the bacterium reflected its biochemical phenotype, and several genes potentially associated with pathogenicity were identified. Bacteria with complex growth requirements frequently remain poorly characterized and, as a consequence, their roles in health and disease are unclear. Elucidation of the physiology and identification of genes putatively involved in the metabolism and virulence of S. amnii may lead to a better understanding of the role of this potential pathogen in bacterial vaginosis, preterm birth, and other issues associated with vaginal and reproductive health.